RNF144B抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: TM4 cells, Primary antibody: P08424(RNF144B Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P08424(RNF144B Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P08424(RNF144B Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The RNF144B antibody is a key tool for studying the Ring Finger Protein 144B (RNF144B), an E3 ubiquitin-protein ligase involved in the ubiquitin-proteasome system. RNF144B contains a characteristic RING domain that facilitates substrate recognition and ubiquitination, targeting proteins for degradation or functional modification. It plays roles in regulating apoptosis, DNA damage response, and cellular stress pathways. Dysregulation of RNF144B has been linked to cancers, neurodegenerative disorders, and immune diseases, making it a focus in biomarker and therapeutic research.

RNF144B antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to detect protein expression, localization, and dynamics in tissues or cell lines. These antibodies help elucidate RNF144B's interactions with substrates such as Parkin, BAX, and HSP70. which are critical for understanding its role in ubiquitination cascades. Validated antibodies often undergo specificity tests using knockout controls to ensure reliability.

Recent studies highlight RNF144B's dual roles in cancer—acting as a tumor suppressor in glioblastoma by promoting apoptosis, yet exhibiting oncogenic properties in lung adenocarcinoma via pro-survival signaling. Its involvement in chemoresistance further underscores clinical relevance. Researchers also explore RNF144B's potential as a therapeutic target, with antibodies aiding in drug screening and mechanistic studies. Continued development of high-affinity, species-specific RNF144B antibodies remains vital for advancing both basic and translational research.