HMGCL抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Mouse brain tissue, Mouse fetal tissue, Mouse adrenal gland tissue lysates, Primary antibody: P08401(HMGCL Antibody) at dilution 1/1200, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 second    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08401(HMGCL Antibody) at dilution 1/75. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P08401(HMGCL Antibody) at dilution 1/75. (Original magnification: ×200)    

  
  

**Background of HMGCL Antibodies**

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a mitochondrial enzyme critical in ketogenesis and leucine metabolism, catalyzing the cleavage of HMG-CoA to acetoacetate and acetyl-CoA. Deficiencies in HMGCL activity due to genetic mutations cause a rare autosomal recessive disorder, HMGCL deficiency, characterized by metabolic crises, hypoketotic hypoglycemia, and organic acidemia. Antibodies targeting HMGCL are essential tools for diagnosing this condition, enabling detection of enzyme expression levels in clinical samples.

Beyond metabolic disorders, HMGCL has gained attention in cancer research. Studies suggest its role in tumor metabolism, particularly in cancers reliant on ketone body utilization or the mevalonate pathway. HMGCL antibodies are employed in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess its expression and subcellular localization in tumor tissues. These studies help explore HMGCL's potential as a therapeutic target or biomarker in oncology.

Commercially available HMGCL antibodies are typically raised against specific epitopes of human HMGCL, with cross-reactivity tested in common model organisms. Their applications extend to elucidating HMGCL's interaction networks, post-translational modifications, and tissue-specific expression patterns. Recent research also investigates HMGCL's involvement in signaling pathways linking metabolism to cell proliferation and survival, highlighting its broader physiological and pathological relevance.