ME3抗体—艾普蒂

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue lysate, Primary antibody: P06906(ME3 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 minutes    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P06906(ME3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

ME3 (Malic Enzyme 3), also known as mitochondrial NADP+-dependent malic enzyme, is a metabolic enzyme that catalyzes the decarboxylation of malate to pyruvate, linking the tricarboxylic acid (TCA) cycle with lipid biosynthesis and cellular redox balance. It plays a critical role in energy metabolism, particularly in tissues with high metabolic demands, such as the liver, brain, and certain cancers. Dysregulation of ME3 has been implicated in metabolic disorders, neurodegenerative diseases, and tumor progression, where altered NADPH/NADP+ ratios and lipid metabolism contribute to pathological processes.

ME3-specific antibodies are essential tools for studying its expression, localization, and function in both physiological and disease contexts. These antibodies enable researchers to detect ME3 protein levels via Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF), aiding in the identification of tissue-specific expression patterns or aberrant ME3 activity in cancer cells. Additionally, ME3 antibodies are used in co-immunoprecipitation (Co-IP) assays to investigate protein-protein interactions and regulatory mechanisms.

Recent studies highlight ME3's potential as a biomarker or therapeutic target in cancers, including glioblastoma and colorectal cancer, where its overexpression correlates with poor prognosis and metabolic adaptation. Validating ME3 antibody specificity through knockdown or knockout controls remains crucial for accurate experimental interpretation. Ongoing research continues to explore ME3's multifaceted roles in mitochondrial metabolism and disease pathogenesis.