Recombinant Mouse M-CSF (carrier-free)(Biolegend 576404 ) 新品

维百奥生物库存现货，特价销售Recombinant Mouse M-CSF (carrier-free)！

| 产品信息

品名：Recombinant Mouse M-CSF (carrier-free)

别名：CSF1, CSF-1, MCSF

品牌：Biolegend

货号：576404

规格：25 µg

| 产品描述

M-CSF was first characterized as a glycoprotein that induces monocyte and macrophage colony formation from precursors in murine bone marrow cultures. M-CSF binds CD14+ monocytes and promotes the survival/proliferation of peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, and cytotoxicity for tumor cells as well as induces the synthesis of inflammatory cytokines such as IL-1, TNFα, and IFNγ in monocytes.

Multiple CSF1 mRNA species have been described that arise from alternative splicing in exon 6 and the alternative use of the 3’ end of exons 9 or 10. As a result, two distinct CSF1 protein products are encoded by these transcripts: a cell-surface or membrane-bound form of CSF1 (mCSF1) and a soluble form (sCSF1). Uterine sCSF1 is highly increased during pregnancy. On the contrary, uterine mCSF1 remains low during pregnancy. High levels of M-CSF have been associated to different pathologies such as pulmonary fibrosis and atherosclerosis.

M-CSF binds to its receptor M-CSFR, and this receptor is shared by a second ligand, IL-34. Mouse M-CSF and IL-34 exhibit cross-species specificity, both bind to the human and mouse M-CSF receptors. IL-34 can regulate myeloid development and substitute for CSF-1 in vivo. IL-34 has overlapping but not identical biological activities as M-CSF.

| 产品细节

1）Source

Mouse M-CSF, amino acids Lys33-Glu262 (Accession# NM_001113530.1) was expressed in 293E cells.

2）Molecular Mass

The 251 amino acid recombinant protein has a predicted molecular mass of approximately 28.2 kD. The DTT-reduced and non-reduced protein migrate at approximately 50 kD and 100 kD respectively by SDS-PAGE. The N-terminal contains a His9-(SGGG)2-IEGR-tag.

3）Purity

>98%, as determined by Coomassie stained SDS-PAGE.

4）Formulation

0.22 µm filtered protein solution is in PBS.

5）Storage & Handling：

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.