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  • HT-29人结肠癌复苏细胞保种中心|带STR证书

HT-29人结肠癌复苏细胞保种中心|带STR证书

HT-29 cell
询价 1000000细胞数 起订
2000000细胞数 起订
上海 更新日期:2025-03-03

上海冠导生物工程有限公司

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产品详情:

中文名称:
HT-29人结肠癌复苏细胞保种中心|带STR证书
英文名称:
HT-29 cell
品牌:
ATCC\RCB等
产地:
国外
保存条件:
常温培养或液氮冻存
纯度规格:
HT-29人结肠癌复苏细胞保种中心|带STR证书
产品类别:
化学试剂
种属:
详见产品资料
组织:
详见产品资料
细胞系:
详见产品资料
细胞形态:
详见产品资料
生长状态:
详见产品资料
靶点:
详见产品资料
应用:
详见产品资料

"HT-29人结肠癌复苏细胞保种中心|带STR证书

传代比例:1:2-1:4(首次传代建议1:2)

生长特性:贴壁生长

贴壁消化难题:1,先用PBS 把细胞洗两遍,使瓶内没有血清了,减少对胰酶的中和,然后用新配的0.25%的胰酶加入3ml左右,放在37度,然后可以在细胞有些消化下来时,拿着瓶口,运用手腕的力量轻轻震荡瓶内体,这样细胞很快就下来了,还不需要吹打,分散也均匀;2,成团、絮状:消化里加入eda可以减少细胞成团的现象,血清可以终止胰酶的作用,如果是进口血清的话也能终止eda的作用。用胰酶消化后胰酶可以倒掉,也可以不倒,直接加血清终止,如果消化中加入了eda的话,就要将消化倒干净,如果细胞贴壁要求不是很严格的话,一般不需要进行离心。鼻咽癌细胞的贴壁能力很强,用0.5%胰酶(含0.1%EDA)一般要消化12~15min。用PBS洗涤时要洗净残余的培养基,加入胰酶后在培养箱中消化(避免细胞室温下受损以及在此温度时胰酶活性Zui强)至细胞收缩变圆(可显微镜下观察)且有少许细胞脱落(有流下来的趋势),随后立即弃去胰酶(如果脱落的细胞很多且需要大量细胞实验,则不能弃去胰酶),加入培养基仔细吹打(不能用无血清培养基或者PBS替代,否则细胞聚集成团块或絮状)。一般我都离心一次弃去上清(去除残留的胰酶及漂浮的死细胞或细胞碎片);消化过度:马上用培养基中和,用吸管吹打细胞,收集全部的细胞到以无菌的离心管中800RPM 3分钟。弃上清,用全培重悬,换新的培养瓶继续培养,状态不HAO的细胞在培养的过程中会死亡脱落,在换的时候可以清除掉!

换液周期:每周2-3次

KU1919 Cells;背景说明:膀胱癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MC9细胞、NMC-G1细胞、Panc 04.03细胞

MGHU3 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:MOLT.4细胞、WM-115细胞、NCIH1963细胞

Karpas 299 Cells;背景说明:间变性大细胞淋巴瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:HNE-1细胞、AML-12细胞、RINm5F细胞

HT-29人结肠癌复苏细胞保种中心|带STR证书

背景信息:该细胞是1964年由FoghJ用移植培养方法和含15%FBS的F12培养从原发性肿瘤分离的。近来,已建株的培养细胞用含血清的McCoy's5a培养基培养。该细胞系在裸鼠中成瘤,也能在类固醇处理的地鼠中成瘤。该细胞可合成IgA、A、TGFβ结合蛋白和黏素;表达尿激酶受体,但没有检测到血浆酶原活性;不表达CD4,但细胞表面表达半乳糖神经酰胺(HIV的可能替代受体)。该细胞系癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos阳性;p53基因过表达,并且在273位密码子处发

公司细胞系形态漂亮、增殖倍数高、纯度高、功能性强,细胞培养就跟养孩子一个样。养孩子要喂奶,养细胞要加补液,都需要在前期补充足够的营养,初始状态的细胞或刚刚复苏的细胞还要适量加入血清或细胞因子来帮助它们的存活增殖,如果营养物质缺乏,细胞就会不生长甚至死亡。养孩子要从小培养学习,养细胞也得培养宝宝顺利生下来,你会经常抚摸他,给他看各种颜色,刺激他的五感。细胞也是一样,分离后的细胞需要使用特定的细胞因子进行活化、增殖。另外加入因子的种类、因子的浓度、加入时间、加入顺序都会影响细胞最终的结果。养孩子最怕孩子生病,养细胞最怕被污染,平时你会仔细观察宝宝是否呕吐、是否突然哭闹,猜测宝宝是否生病了。对于细胞,我们也需要时刻进行观察的,假如培养液浑浊(污染了),则需要换液后加抗生素;假如细胞增殖不明显,形态变差,则可能是因为营养不足了,对贴壁细胞可以消化后重新用新的培养基接种并加倍加入细胞因子含量;对悬浮细胞增殖能力不强的,则不着急补液,只是先补加血清、细胞因子看是否可以好转。培养时还得全程在无菌的环境,一个小小的偏差,细胞就会死亡。

产品包装:复苏发货:T25培养瓶(一瓶)或冻存发货:1ml冻存管(两支)

来源说明:细胞主要来源ATCC、ECACC、DSMZ、RIKEN等细胞库

WERI Rb-1 Cells;背景说明:WERI-Rb-I细胞株是1974年R.M. McFall 和 T.W. Sery建立的两株人眼癌细胞系中的一株。 细胞能在Difco Bacto-Agar中存活但不形成克隆。 扫描电镜显示在表面囊泡,板状伪足和微绒毛在数量上和频率上的改变。 细胞分化研究,肿瘤治疗的动物模型和生化评价都涉及这株细胞。;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:圆形细胞聚集成葡萄状;相关产品有:LY细胞、SW1116细胞、U-343 MG细胞

RGC6 Cells;背景说明:胶质细胞株C6是由Benda等用N-nitrosomethylurea诱导的大鼠胶质瘤克隆,并经过一系列的体外培养和动物传代交替后建成的。 当细胞从低密度生长到满瓶时,S-100产量增加10倍。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:SF-295细胞、MFE 296细胞、KYSE 180细胞

Large Cell Lung Cancer-103H Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:WPMY-1细胞、A2780CP细胞、NCI-SNU-423细胞

WERI Cells;背景说明:WERI-Rb-I细胞株是1974年R.M. McFall 和 T.W. Sery建立的两株人眼癌细胞系中的一株。 细胞能在Difco Bacto-Agar中存活但不形成克隆。 扫描电镜显示在表面囊泡,板状伪足和微绒毛在数量上和频率上的改变。 细胞分化研究,肿瘤治疗的动物模型和生化评价都涉及这株细胞。;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:圆形细胞聚集成葡萄状;相关产品有:OV3121细胞、AHH1细胞、HAC-84细胞

HT-29人结肠癌复苏细胞保种中心|带STR证书

物种来源:人源、鼠源等其它物种来源

形态特性:上皮细胞样

【胰蛋白酶消化操作】1)加入消化:小心吸出旧培养,用PBS清洗(冲洗),加入适量消化(胰蛋白酶),注意消化的量以盖住细胞ZuiHAO,Zui佳消化温度是37℃;2)显微镜下观察细胞:倒置显微镜下观察消化细胞,若胞质回缩,细胞之间不再连接成片,表明此时细胞消化适度;(3)吸弃消化加入培养:弃去胰蛋白酶,注意更换吸管,加入新鲜的培养。【吹打分散细胞操作】1)吹打制悬:用滴管将已经消化细胞吹打成细胞悬;2)吸细胞悬入离心管:将细胞悬吸入10ml离心管中;3)平衡离心:平衡后将离心管放入台式离心机中,以1000转/分钟离心6-8分钟;4)弃上清,加入新培养:弃去上清,加入2ml培养,用滴管轻轻吹打细胞制成细胞悬。【分装稀释细胞操作】1)显微镜下观察细胞:倒置显微镜下观察细胞量,必要是计数。注意密度过小会影响传代细胞的生长,传代细胞的密度应该不低于5×105/ml。Zui后要做HAO标记;2)分装:将细胞悬吸出分装至2~3个培养瓶中,加入适量培养基旋紧瓶盖;3)继续培养:用酒精棉球擦拭培养瓶,适当旋松瓶盖,放入CO2培养箱中继续培养。传代细胞2小时后开始贴附在瓶壁上。

KYSE 140 Cells;背景说明:食管鳞癌细胞;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:KU19-19细胞、SG231细胞、LC1/Sq细胞

NCI-SNU-1 Cells;背景说明:详见相关文献介绍;传代方法:2-3天换液1次。;生长特性:悬浮聚集;形态特性:上皮细胞;相关产品有:HCC-78细胞、MGSMC细胞、MH7A细胞

NCI-H1373 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:4传代;2-3天换液1次。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:HLMVEC细胞、Colon 38细胞、RIN Cl-5F细胞

231-luc Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:BTT739细胞、HCC-2185细胞、RPE1-hTERT细胞

LIM1215 Cells;背景说明:结直肠癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HCC44细胞、HL60细胞、MDAMB468细胞

Leukemic 1210 Cells;背景说明:该细胞源于用0.2%甲基胆蒽(溶解)涂抹雌性小鼠的皮肤诱发的肿瘤,鼠痘病毒阴性。;传代方法:1:2传代;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:NCI-H2108细胞、H-1693细胞、Co320细胞

MC-116 Cells;背景说明:详见相关文献介绍;传代方法:每周换液2-3次。;生长特性:悬浮生长 ;形态特性:淋巴母细胞样;相关产品有:SBC3细胞、NPA87-1细胞、H-510细胞

SF-763 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:CCRF SB细胞、RH8994细胞、L cell line细胞

GM00637B Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:Neukoplast细胞、L-M(TK-)细胞、alphaTC1 Clone 6细胞

Spodoptera frugiperda clone 9 Cells;背景说明:详见相关文献介绍;传代方法:1:5传代;2-3天换液1次。;生长特性:混合生长;形态特性:上皮样;相关产品有:IPLB-Sf21细胞、VP 267细胞、KBM5细胞

DAN-G Cells;背景说明:胰腺癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:aNK细胞、MC 3T3-E1细胞、Ramos-2G6-4C10细胞

MA-c Cells;背景说明:星形胶质 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:H69/P细胞、UMUC-3细胞、Alexander cells细胞

A.704 Cells;背景说明:详见相关文献介绍;传代方法:1:3—1:4传代,每周换液2-3次;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:HEK293-EBNA细胞、16HBE140细胞、HSC-2细胞

TK 10 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:6传代;2-3天换液1次。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:MC-116细胞、AG06814-N细胞、CAL851细胞

NCIH1869 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:4传代;每周换液2次。;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:H2.35细胞、NCI-H345细胞、NCI-H-295细胞

CCD-1112sk Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:Renal Carcinoma细胞、Vertebral Cancer of the Prostate细胞、H727细胞

MDA-MB-134 VI Cells;背景说明:该细胞1973年由R. Cailleau建系,源自74岁乳腺导管癌女性患者的胸腔积液,细胞生长缓慢,松散贴壁,生长过程中会脱落到培养基,不会汇合,过表达FGF受体;传代方法:1:2—1:4传代,每周换液2—3次;生长特性:松散贴壁生长;形态特性:上皮细胞样;相关产品有:G292细胞、Centre Antoine Lacassagne-33细胞、Plaepi 34细胞

HT-29人结肠癌复苏细胞保种中心|带STR证书

Abcam HEK293T DAPK3 KO Cells(提供STR鉴定图谱)

AG09861 Cells(提供STR鉴定图谱)

BayGenomics ES cell line NPX269 Cells(提供STR鉴定图谱)

BayGenomics ES cell line XC499 Cells(提供STR鉴定图谱)

BT-20/CMV-Luc Cells(提供STR鉴定图谱)

COS-1-InVitrus Cells(提供STR鉴定图谱)

DA04032 Cells(提供STR鉴定图谱)

DLD-1 ATG7(+/-) Cells(提供STR鉴定图谱)

GM03030 Cells(提供STR鉴定图谱)

HLFa Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:4传代;每周换液2次。;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:H1563细胞、SNU-398细胞、H2196细胞

Mouse Forestomach Carcinoma Cells;背景说明:胃癌;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MCA 205细胞、GM00346B细胞、Capan-1细胞

COLO_320DM Cells;背景说明:该细胞可产生5-羟色胺、去甲、、ACTH和甲状旁腺素。角蛋白、波形蛋白弱阳性。培养条件: RPMI 1640  10%FBS;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮+贴壁;形态特性:淋巴细胞;相关产品有:HANK-1细胞、SUDHL-8细胞、H22细胞

H1581 Cells;背景说明:详见相关文献介绍;传代方法:每周换液2次。;生长特性:混合型;形态特性:上皮样;相关产品有:HSC-6细胞、L 132细胞、SPDC-CCL141细胞

OVCA432 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:KU 19-19细胞、REC-1细胞、H-838细胞

3-LL Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:详见产品说明书;相关产品有:CCD 966 SK细胞、SRS-82细胞、SW-954细胞

558-D100,10,1 Cells(提供STR鉴定图谱)

CAL 62 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:NTC200细胞、TB1 Lu细胞、SP2-0-Ag14细胞

Lu-165 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:HEY A8细胞、HuO9细胞、HCC 70细胞

BXPC3 Cells;背景说明:这个细胞株不表达囊肿性纤维化跨膜电导调节子(CFTR)。CFTR阳性的细胞株是Capan-1(ATCCHTB-79)。;传代方法:消化5-10分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:ARPE-19细胞、Roswell Park Memorial Institute 6666细胞、H-7721细胞

OCI Ly1 Cells;背景说明:弥漫大B淋巴瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:NCIH1437细胞、P3X63 Ag8.653细胞、SUDHL-2细胞

769-P Cells;背景说明:该细胞系1975年建系,源自一位63岁白人女性的初期透明细胞腺癌组织,细胞呈圆形且边界不清,核浆比大,有微绒毛及桥粒。该细胞可在软琼脂上生长。 ;传代方法:1:4—1:12传代,2—3天换液一次;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:143B细胞、MA 104细胞、RBE细胞

PANC0504 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:ATN1细胞、HIT细胞、SK-MEL-24细胞

3T3-F442A Cells;背景说明:脂肪前体细胞;雄性;Swiss albino;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:rRTEC细胞、SK-LU-1细胞、Panc 05.04细胞

DHL-8 Cells;背景说明:弥漫大B淋巴瘤;腹腔积液转移;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:NS-1细胞、CAL-51细胞、PTK 2细胞

GM17687 Cells(提供STR鉴定图谱)

HAP1 HSPA1L (-) 2 Cells(提供STR鉴定图谱)

TSUpr1 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:SNU761细胞、UCLA NPA871细胞、KG1细胞

NCI-HUT-596 Cells;背景说明:详见相关文献介绍;传代方法:1:4-1:8传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:675T细胞、A 253细胞、CWR22Rv1细胞

2BS Cells;背景说明:胚肺;成纤维细胞;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:alphaTC1 Clone 6细胞、NCI-H2030细胞、H69细胞

SK-OV-3 Cells;背景说明:SK-OV-3由G.Trempe和L.J.Old在1973年从卵巢肿瘤病人的腹水分离得到。 此细胞对肿瘤坏死因子和几种细胞毒性药物包括白喉毒素、顺铂和阿霉素均耐受。 在裸鼠中致瘤,且形成与卵巢原位癌一致的中度分化的腺癌。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:上皮细胞样;相关产品有:WM-239-A细胞、TCMK1细胞、NCIH2405细胞

CAL 39 Cells;背景说明:外阴鳞癌细胞;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MM1S细胞、NCI-H1522细胞、MMAc.SF细胞

SUM-102 Cells;背景说明:乳腺癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:H-1651细胞、NRK 49F细胞、SKN-AS细胞

no-11 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:MiaPaca.2细胞、KMB-17细胞、HRIF细胞

KU19-19 Cells;背景说明:膀胱癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:LTEPa2细胞、GalK1细胞、PLB-985细胞

HPS0159 Cells(提供STR鉴定图谱)

JHU170i Cells(提供STR鉴定图谱)

MDA-BoM-1833 Cells(提供STR鉴定图谱)

ND33950 Cells(提供STR鉴定图谱)

PR01238 Cells(提供STR鉴定图谱)

U2OS ADRA1B DAG-Nomad Cells(提供STR鉴定图谱)

UMANe002-A-5 Cells(提供STR鉴定图谱)

HAP1 TSC2 (-) 1 Cells(提供STR鉴定图谱)

JM-1 Cells;背景说明:详见相关文献介绍;传代方法:换液2-3次一周;生长特性:悬浮生长 ;形态特性:淋巴母细胞样;相关产品有:RPMI No. 1846细胞、Clone Y-1细胞、H7721细胞

COC1/CDDP Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:UCLA RO-81A-1细胞、IFRS1细胞、DHL-6细胞

MKN 45 Cells;背景说明:该细胞系由S Akiyama建立,源于一位35岁患有印戒细胞癌的女性的胃淋巴结。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁+悬浮;形态特性:淋巴母细胞;相关产品有:hRPTC细胞、SK-MEL-2-LUC细胞、T2细胞

Raji Cells;背景说明:Raji细胞由PulvertaftRJV于1963年从一位11岁黑人男孩的左上颌骨的Burkitt淋巴瘤中分离建立的,是第一个人类造血系统的连续传代细胞,为B细胞起源。该细胞中含有EBV,需要在二级生物安全柜中操作;可作转染宿主。;传代方法:维持细胞浓度在4×105/ml-3×106/ml;根据细胞浓度每2-3天补液1次。;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:VAESBJ细胞、Hs737T细胞、HGC-27细胞

251MG Cells;背景说明:U-251 MG分离至一位患者的胶质母细胞瘤组织。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:成纤维细胞样;相关产品有:Hs-578T细胞、IMCD-3细胞、HN 4细胞

251MG Cells;背景说明:U-251 MG分离至一位患者的胶质母细胞瘤组织。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:成纤维细胞样;相关产品有:Hs-578T细胞、IMCD-3细胞、HN 4细胞

H647ell Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:6传代;每周换液2次。;生长特性:贴壁生长;形态特性:详见产品说明书;相关产品有:SMMC-7721细胞、C3H10T1/2细胞、OCIAML4细胞

OCIAML3 Cells;背景说明:急性髓系白血病;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:BetaTC6细胞、GM2132细胞、HS-5细胞

V79-1 Cells;背景说明:肺;自发永生;雄性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MM96L细胞、Capan1细胞、HEY-A8细胞

CCD966SK Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:KYSE-150细胞、Hs821T细胞、YD38细胞

231-luc Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:BTT739细胞、HCC-2185细胞、RPE1-hTERT细胞

231-luc Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:BTT739细胞、HCC-2185细胞、RPE1-hTERT细胞

MDA.MB.231 Cells;背景说明:MDA-MB-231来自患有转移乳腺腺癌的51岁女病人的胸水。在裸鼠和ALS处理的BALB/c小鼠中,它能形成低分化腺癌(III级)。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:PANC 203细胞、VP267细胞、KY70细胞

ECV 304 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:SK-MEL24细胞、NCI-H596细胞、THLE-2细胞

UM-UC-14 Cells;背景说明:肾癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:CF-1 MEF细胞、GL261细胞、ZYM-DIEC02细胞

SKO-007 clone J3 Cells(提供STR鉴定图谱)

H1385 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:MM.1S细胞、MD Anderson-Metastatic Breast-175-VIII细胞、HeLa229细胞

K299 Cells;背景说明:间变性大细胞淋巴瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:CL34细胞、H-2347细胞、NK-92细胞

Tregs Cells;背景说明:调节性T Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:Epstein-Barr-3细胞、SCC9细胞、SKNBE2细胞

293-H Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:NCI/ADRRES细胞、SKNAS细胞、Vero E-6细胞

BNL CL2 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长 ;形态特性:详见产品说明书;相关产品有:BT 20细胞、MDA2B细胞、Hs-600-T细胞

Pa14C Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:DLD 1细胞、HL7702细胞、RPMI no. 8226细胞

U-87MG Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:D341Med细胞、B16/BL6细胞、TT细胞

SCL-II Cells;背景说明:皮肤鳞癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:C41细胞、LL/2(LLc1)细胞、CCFSTTG1细胞

SNU182 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:U266细胞、CC-LP-1细胞、hTERT-RPE1细胞

Panc 05.04 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:KMST6细胞、C2C12细胞、U-251 MG细胞

NCIH1092 Cells;背景说明:详见相关文献介绍;传代方法:随细胞的密度而增加;生长特性:悬浮生长;形态特性:详见产品说明书;相关产品有:RCC-JF细胞、SK_N_FI细胞、MHCCLM3细胞

UMNSAH-DF-1 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:成纤维母细胞样;相关产品有:U343MG细胞、SW1116细胞、OSKM-1细胞

SVG p12 Cells;背景说明:星形胶质细胞;SV40转化;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:H1993细胞、Clone Y1细胞、KU-812细胞

NCI-H211 Cells;背景说明:详见相关文献介绍;传代方法:3-4天换液1次。;生长特性:悬浮生长;形态特性:详见产品说明书;相关产品有:H-1693细胞、RCC-4细胞、UMUC-3细胞

HT-29人结肠癌复苏细胞保种中心|带STR证书

BayGenomics ES cell line RRK254 Cells(提供STR鉴定图谱)

BayGenomics ES cell line XL961 Cells(提供STR鉴定图谱)

Glut1(D4417-14) Cells(提供STR鉴定图谱)

OKT 8 Cells(提供STR鉴定图谱)

ZUE12 Cells(提供STR鉴定图谱)

MCW070i-40002330 Cells(提供STR鉴定图谱)

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Fogh J., Fogh J.M., Orfeo T.

One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.

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Absence of HeLa cell contamination in 169 cell lines derived from human tumors.

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Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.

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Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.

Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.

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Differential repair of 1-(2-chloroethyl)-3-(4-methylcyclohexyl)-1- nitrosourea-induced DNA damage in two human colon tumor cell lines.

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Growth enhancing property of human monocytes from normal donors and cancer patients.

Immunology 37:247-252(1979)


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Day R.S. 3rd, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Lubiniecki A.S., Girardi A.J., Galloway S.M., Bynum G.D.

Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.

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Isolation of a cellular subpopulation from a human colonic carcinoma cell line.

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Day R.S. 3rd, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Mattern M.R.

Human tumor cell strains defective in the repair of alkylation damage.

Carcinogenesis 1:21-32(1980)


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HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.

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Pathol. Oncol. R"



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