MCF7[MCF-7]人乳腺癌细胞全年复苏|已有STR图谱

"MCF7[MCF-7]人乳腺癌细胞全年复苏|已有STR图谱

传代比例：1:2-1:4(首次传代建议1:2)

生长特性：贴壁生长

【细胞培养经验分享】启蒙老师的重要性：一般进实验室都有师兄师姐带着做，他们就是你做细胞的启蒙老师。他们的操作手法、细节、理论讲解就成了你操作的准则，如营养液、细胞瓶的摆放位置、灭菌处理程序、开盖手法、细胞吹打手法等等。要学会他们的正确操作，在第一次的时候就要重视。像养孩子一样养细胞，细胞有时真的很脆弱，最好每天都去看看它，以防止出现培养箱缺水、缺二氧化碳、停电、温度不够等异常现象，也好及时解决这些意外，避免重复实验带来的更大痛苦。好细胞要及时保种：细胞要分批传代，这样即使有一批出了问题，还有一批备用的。像后者一般人可能不容易做到。但这是我血的教训，有一次细胞污染了，全军覆没。当时可后悔没有保种。细胞跟人一样，不同的细胞，培养特性是不一样的。培养过程中要细细体会，不同细胞系使用不同的培养基和血清。

换液周期：每周2-3次

NCI-H2228 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代，每周２-３次。;生长特性：贴壁生长;形态特性：详见产品说明书;相关产品有：HEM细胞、CaES-17细胞、Jiyoye(P-2003)细胞

PL-11 Cells;背景说明：详见相关文献介绍;传代方法：１：２传代;生长特性：贴壁生长;形态特性：上皮样;相关产品有：PA1细胞、RINm-5F细胞、B 95.8细胞

4175 Cells;背景说明：乳腺癌;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：OVCAR433细胞、MDCK II细胞、Human Melanoma Cell Bowes细胞

背景信息：MCF-７细胞保留了多个分化了的乳腺上皮的特性，包括：能通过胞质雌激素受体加工雌二醇并能形成圆形复合物（domes）。该细胞含有Tx-４癌基因。肿瘤坏死因子α（TNFalpha）可以抑制MCF-７细胞的生长。抗雌激素处理细胞能调变IGFBP＇S的分泌。

MCF7[MCF-7]人乳腺癌细胞全年复苏|已有STR图谱

产品包装：复苏发货：T25培养瓶(一瓶)或冻存发货：1ml冻存管(两支)

贴壁细胞的传代培养，详细步骤如下：首先倒掉培养基，在这一步骤可以收集一些细胞上清做支原体检测；加入胰蛋白酶，一般T25是加2mL，盖好瓶盖，摇晃T25培养瓶，使胰蛋白酶均匀覆盖在细胞表面，放入培养箱2-3min，期间可在显微镜下观察，看到大部分细胞变圆，即可放入超净台，加入2倍的完全培养基，这里就是加4mL培养基，终止消化；将含有胰蛋白酶，细胞和培养基一起转移到离心管中，1000rpm/3min离心，去掉上清；新鲜的完全培养基重悬，根据细胞的生长特性和后续的实验需求进行传代，比如我养的Hepa1-6就长的比较快，不是着急用的话，我就会按1E6个细胞/T75培养瓶进行传代；但如果后两天要用，就会适当多传一点；还可通过显微镜计数后，直接用于细胞铺板，继续后续的实验。

TE8 Cells;背景说明：详见相关文献介绍;传代方法：消化３-５分钟。１：２。３天内可长满。;生长特性：贴壁生长;形态特性：上皮样;相关产品有：RASMC细胞、NKM-1细胞、PLA-801D细胞

WiDr-TC Cells;背景说明：结肠癌；女性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：Los Angeles Prostate Cancer-4细胞、SUM-190PT细胞、WIL2 Secreting细胞

U031 Cells;背景说明：详见相关文献介绍;传代方法：１：３-１：６传代；２-３天换液１次。;生长特性：贴壁生长;形态特性：上皮样;相关产品有：HEL299细胞、NCIH1385细胞、CCF-STTG1细胞

4F2 [Mouse hybridoma against fenitrothion] Cells(提供STR鉴定图谱)

来源说明：细胞主要来源ATCC、ECACC、DSMZ、RIKEN等细胞库

物种来源：人源、鼠源等其它物种来源

MCF7[MCF-7]人乳腺癌细胞全年复苏|已有STR图谱

形态特性：上皮细胞样

贴壁细胞消化传代时通常采用两种方法：一、加入胰酶等细胞脱落后，再加培养基中止胰酶作用，离心传代；二、加入胰酶后，镜下观察待细胞始脱落时，弃胰酶，加培养分瓶。但前者太麻烦，而后者有可能对细胞施加胰酶选择，因为总是贴壁不牢的细胞先脱落，对肿瘤细胞来说，这部分细胞有可能是恶性程度较GAO的细胞亚群。一种简单的消化传代方法。加入PBS洗去血清或加入胰酶先中和血清的作用(３０s)，弃之，再加入适量胰酶作用１０s-４０s(根据细胞消化的难易程度)，弃之，这样依赖残余的胰酶就可将细胞消化单细胞。对于较难消化的细胞，可以用２%利多卡因消化５-８分钟，然后再弃去，加培养基吹打也可以，对细胞的影响不大。不用PBS也不用Hanks洗，只要把旧培养吸的干净一点，直接加酶消化应该不会有什么问题。弃培养后，用０.０４%的EDA冲洗一次，再用１/４v的０.０４%的EDA室温孵育５min，弃取大部分EDA，加入与剩余EDA等量的胰酶(预热)总体积１/１０v。消化到有细胞脱落。不过有人说EDA对细胞不HAO，有证据吗？培养的BASMC：倒掉旧培养加入少量胰酶冲一下，倒掉再加入０.１２５-０.２５%胰酶约６-１０滴或１ml(２５ml bole)消化再加入适量新培养基中和，并分瓶这种方法简单、省事；效果很HAO并且不损失细胞！

BNL 1ME A.7R.1 Cells;背景说明：肝；上皮细胞；BALB/c;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：HCC2218细胞、SW 1573细胞、COLO 684细胞

SW-1463 Cells;背景说明：详见相关文献介绍;传代方法：１：３—１：８传代，每周换液１-２次;生长特性：贴壁生长;形态特性：上皮细胞;相关产品有：HO1-N-1细胞、MCF.7细胞、BEAS2B细胞

LNCaP-Clone-FGC Cells;背景说明：人前列腺癌细胞LNCaP克隆FGC是从一位５０岁白人男性(血型B+)的左锁骨淋巴结针刺活检中分离，该患者经确诊为前列腺癌转移。 这株细胞对５-α-二睾酮(生长调节子和酸性脂酶产物)有响应。这株细胞并不形成一致的单层，而是形成集落，在传代时可以用滴管反复吹吸打碎。它们仅仅轻轻地吸附在基底上，不形成汇合，很快使培养基变酸。生长很慢。传代后４８小时内不应扰动。当培养瓶封包后，多数细胞从培养瓶底分离，悬浮在培养基中。收到后，在通常培养单层细胞的条件下培养２４到４８小时，以合细胞再贴壁。;传代方法：消化３-５分钟。１：２。３天内可长满。;生长特性：贴壁生长;形态特性：上皮细胞;相关产品有：H1954细胞、3T6 Swiss Albino细胞、Tj-905细胞

BE2-C Cells;背景说明：神经母细胞瘤；骨髓转移；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：HCT/FU细胞、U-2-OS细胞、NCI-841细胞

GM00637F Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁或悬浮，详见产品说明书部分;形态特性：详见产品说明书;相关产品有：TMD-8细胞、H766T细胞、X63-Ag8细胞

SN12-PM6 Cells;背景说明：肾癌；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：Panc08.13细胞、CNE-1细胞、108CC15细胞

CCLP-1 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁或悬浮，详见产品说明书部分;形态特性：详见产品说明书;相关产品有：NPC-TW-039细胞、CORL51细胞、A20细胞

Balb/3T3-4-Cl31 Cells;背景说明：胚胎；成纤维；自发永生；雄性；BALB/c;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：PL-5细胞、Tb1.Lu细胞、CHG5细胞

SBC3 Cells;背景说明：小细胞肺癌；骨髓转移；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：L-M[TK-]细胞、RPE1细胞、WERI Rb-1细胞

H125 Cells;背景说明：腺鳞状肺癌；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：MOLM16细胞、LLC-WRC 256细胞、RPPVEC细胞

PG-4 S+L- Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁或悬浮，详见产品说明书部分;形态特性：详见产品说明书;相关产品有：PLMVEC细胞、SW-780细胞、HSC-3细胞

HEL-92.1.7 Cells;背景说明：详见相关文献介绍;传代方法：每周２-３次。;生长特性：悬浮生长;形态特性：成淋巴细胞;相关产品有：H-35 Reuber细胞、SW620细胞、H-1836细胞

U-138 MG Cells;背景说明：星形细胞瘤；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：NCIH196细胞、A549/DDP细胞、VMRCRCZ细胞

16HBE140 Cells;背景说明：详见相关文献介绍;传代方法：１：２传代;生长特性：贴壁生长　;形态特性：详见产品说明书;相关产品有：NCI-SNU-475细胞、LCLC-103H细胞、BTI-TN5B1-4细胞

QGY Cells;背景说明：该细胞系来自３５岁女性的肝癌。染色体数目变化大，异倍体多。免疫荧光间接法AFP阳性反应。异体移植能力强。亚显微结构方面，核与细胞质的比例高，大的多形核和多种细胞器亚显微结构改变。在ConA作用下凝集。倍增时间约为２０.５小时。用Northernblot方法，未能检测到细胞中１.３kbLFIRE-１/HFREP-１mRNA的表达。;传代方法：消化３-５分钟，１：２,３天内可长满;生长特性：贴壁生长;形态特性：上皮样;相关产品有：CCD-1112sk细胞、IPLB-SF-21细胞、HCC-4006细胞

SN12C Cells;背景说明：详见相关文献介绍;传代方法：２x１０^４ cells/ml;生长特性：贴壁生长;形态特性：上皮细胞样;相关产品有：H727细胞、D283-MED细胞、TJ905细胞

MV 4;11 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁或悬浮，详见产品说明书部分;形态特性：详见产品说明书;相关产品有：KYSE 140细胞、CV-1.K细胞、RAW2647细胞

5HL2-B Cells(提供STR鉴定图谱)

Abcam K-562 PDHB KO Cells(提供STR鉴定图谱)

AP38S Cells(提供STR鉴定图谱)

BayGenomics ES cell line RRM013 Cells(提供STR鉴定图谱)

BayGenomics ES cell line XN258 Cells(提供STR鉴定图谱)

cAMP Hunter CHO-K1 GIPR Gs Cells(提供STR鉴定图谱)

DA00703 Cells(提供STR鉴定图谱)

Detroit 98/AG Cells(提供STR鉴定图谱)

GM02631 Cells(提供STR鉴定图谱)

BT549 Cells;背景说明：该细胞１９７８年由W.G.Coutinho和E.Y.Lasfargues建系，源自一位７２岁患有乳腺导管癌的白人女性，来源组织包括乳头及浸润导管。该细胞形态包括上皮样细胞及多核巨细胞，可分泌一种粘性物质。;传代方法：１：２传代;生长特性：贴壁生长;形态特性：上皮样;相关产品有：HEC1-A细胞、U343MG细胞、DMS79细胞

HCC2185 Cells;背景说明：转移性小叶乳腺癌；胸腔积液转移；女性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：BEL-7402细胞、CCD-33Co细胞、B16/F0细胞

WM-266-mel Cells;背景说明：详见相关文献介绍;传代方法：１：２传代;生长特性：贴壁生长;形态特性：详见产品说明书;相关产品有：H2066细胞、SK-RC-39细胞、SPC-A1细胞

NOZAWA Cells;背景说明：患者有癌性腹膜炎。细胞为中等分化的管状胆囊癌。会分泌AFP和CEA。倍增时间４８小时，板植率１４-１９%。细胞可在裸鼠中成瘤，形态与原发肿瘤相似。;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：上皮细胞样;相关产品有：ME180细胞、NCI-SNU-878细胞、HepaRG细胞

RBMEC Cells;背景说明：脑微血管；内皮 Cells;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：mRTEC细胞、Hs 729细胞、Ramos(RA1)细胞

NB-9 Cells;背景说明：详见相关文献介绍;传代方法：１：１０ １：５０每２ - ３周；每周换液２-３次。;生长特性：贴壁生长;形态特性：成神经细胞;相关产品有：LYR细胞、HEM细胞、HEK-AD 293细胞

HS5 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：上皮细胞样;相关产品有：huH 1细胞、PLB985细胞、COLO829细胞

SF126 Cells;背景说明：该细胞来源于星形胶质细胞瘤；胶质纤维酸性蛋白（GFAP）阴性；可以特异地结合β-内啡肽。;传代方法：１：３传代；３-４天１次。;生长特性：贴壁生长;形态特性：成纤维细胞;相关产品有：KMH-2细胞、MCA 205细胞、MKN 7细胞

MCF7[MCF-7]人乳腺癌细胞全年复苏|已有STR图谱

H1648 Cells;背景说明：详见相关文献介绍;传代方法：１：３-１：６传代;生长特性：贴壁生长;形态特性：上皮细胞;相关产品有：HFSF细胞、Wills Eye Research Institute-Retinoblastoma-1细胞、HBE细胞

KM932 Cells;背景说明：B淋巴细胞；EBV转化;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：悬浮;形态特性：详见产品说明书;相关产品有：T-24细胞、Caco-2细胞、HRC-99细胞

MOLT-3 Cells;背景说明：急性T淋巴细胞白血病；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：悬浮;形态特性：详见产品说明书;相关产品有：HKBML细胞、NE1-E6E7细胞、BEL-7402细胞

B-104 Cells;背景说明：神经母细胞瘤；BDIX;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：COLO-320DM细胞、SaOS细胞、RMS 13细胞

hCMEC/D3 Cells;背景说明：脑微血管；内皮 Cells;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：SACC-83细胞、EBTr细胞、MN9D细胞

hCMEC/D3 Cells;背景说明：脑微血管；内皮 Cells;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：SACC-83细胞、EBTr细胞、MN9D细胞

Panc 4.03 Cells;背景说明：详见相关文献介绍;传代方法：１：２传代;生长特性：贴壁生长;形态特性：上皮样;相关产品有：WEHI-3细胞、RTE细胞、NCI-H2452细胞

GM28211 Cells(提供STR鉴定图谱)

HAP1 PCDH9 (-) 2 Cells(提供STR鉴定图谱)

253JB-V Cells;背景说明：膀胱癌；淋巴结转移；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：OV2008细胞、HSC-6细胞、NCI-HUT-520细胞

HEK293E Cells;背景说明：详见相关文献介绍;传代方法：１：４-１：１０传代；每周２次。;生长特性：贴壁生长;形态特性：上皮细胞样;相关产品有：RPMI-7951细胞、H920细胞、Tn-5B1-4细胞

LAC Cells;背景说明：肺癌;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：SGC 7901细胞、CFSC-8B细胞、Panc 5.04细胞

LuCL4 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁或悬浮，详见产品说明书部分;形态特性：详见产品说明书;相关产品有：CD 18细胞、BC-025细胞、Baby Hamster Kidney 21细胞

GM00215A Cells;背景说明：肺；自发永生；雄性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：HOSEpiC细胞、H2122细胞、GM02219D细胞

U-226AR1 Cells;背景说明：骨髓瘤；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：悬浮;形态特性：详见产品说明书;相关产品有：H-1417细胞、U-937细胞、WML2细胞

HEK/293 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁或悬浮，详见产品说明书部分;形态特性：详见产品说明书;相关产品有：VMM5A细胞、Human Corneal Epithelial cells-Transformed细胞、L-M[TK-]细胞

SNU484 Cells;背景说明：详见相关文献介绍;传代方法：１：２传代;生长特性：贴壁或悬浮，详见产品说明书部分;形态特性：详见产品说明书;相关产品有：Hs819T细胞、NCIH64细胞、OV-2008细胞

HS306 Cells(提供STR鉴定图谱)

KOLF2.1J LRRK2 R1441C SNV/SNV Cells(提供STR鉴定图谱)

MNNG/HOS/DXR1000 Cells(提供STR鉴定图谱)

NUIGi050-B Cells(提供STR鉴定图谱)

RG-236 Cells(提供STR鉴定图谱)

TMPi005-B Cells(提供STR鉴定图谱)

UD-SCC-7A Cells(提供STR鉴定图谱)

HG01325 Cells(提供STR鉴定图谱)

TW01 Cells;背景说明：鼻咽癌;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：WEHI3B细胞、CCD966SK细胞、SKRC-39细胞

38C-13 Cells;背景说明：B淋巴瘤；C３H/eB;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：半贴壁;形态特性：详见产品说明书;相关产品有：MAVER细胞、HT-3细胞、U343细胞

C6661 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：上皮细胞样;相关产品有：MOLP-8细胞、THLE-3细胞、NS1细胞

RAW264.7 Cells;背景说明：单核巨噬细胞白血病；雄性；BALB/c;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：SK MEL 5细胞、U-266细胞、Panc02-H0细胞

BEAS2B Cells;背景说明：从一位非癌个体的正常人支气管上皮病理切片分离出上皮细胞。这些细胞用腺病毒１２-SV４０病毒杂交病毒感染并克隆。DEAS-２B细胞保留了对血清反应进行鳞关分化的能力，并有用于筛选诱导或影响分化及致癌的化学或生物制剂。细胞角蛋白及SV４０抗原染色阳性。;传代方法：消化３-５分钟。１：２。３天内可长满;生长特性：贴壁生长;形态特性：上皮细胞样;相关产品有：JOSK-M细胞、MCA 38细胞、WM-239-A细胞

BEAS2B Cells;背景说明：从一位非癌个体的正常人支气管上皮病理切片分离出上皮细胞。这些细胞用腺病毒１２-SV４０病毒杂交病毒感染并克隆。DEAS-２B细胞保留了对血清反应进行鳞关分化的能力，并有用于筛选诱导或影响分化及致癌的化学或生物制剂。细胞角蛋白及SV４０抗原染色阳性。;传代方法：消化３-５分钟。１：２。３天内可长满;生长特性：贴壁生长;形态特性：上皮细胞样;相关产品有：JOSK-M细胞、MCA 38细胞、WM-239-A细胞

SW 403 Cells;背景说明：详见相关文献介绍;传代方法：１：２—１：６传代，每周换液２-３次;生长特性：贴壁生长;形态特性：上皮细胞;相关产品有：NBL_S细胞、4T1-LUC细胞、A375细胞

HCC-4006 Cells;背景说明：详见相关文献介绍;传代方法：１：４-１：６传代，每周２-３次。;生长特性：贴壁生长;形态特性：上皮细胞样;相关产品有：SNU869细胞、H-841细胞、Suzhou Human Glioma-44细胞

CAOV4 Cells;背景说明：卵巢癌；女性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：G-292, clone A141B1细胞、Hs 766细胞、Panc08.13细胞

G361 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：４传代，２-３天换液１次。;生长特性：贴壁生长;形态特性：上皮细胞;相关产品有：EBNA-293细胞、H-1755细胞、Ly10细胞

BE(2)C Cells;背景说明：神经母细胞瘤；骨髓转移；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：SUM 149PT细胞、NT2/D1细胞、BALB/c 3T3 clone A31细胞

BEAS2B Cells;背景说明：从一位非癌个体的正常人支气管上皮病理切片分离出上皮细胞。这些细胞用腺病毒１２-SV４０病毒杂交病毒感染并克隆。DEAS-２B细胞保留了对血清反应进行鳞关分化的能力，并有用于筛选诱导或影响分化及致癌的化学或生物制剂。细胞角蛋白及SV４０抗原染色阳性。;传代方法：消化３-５分钟。１：２。３天内可长满;生长特性：贴壁生长;形态特性：上皮细胞样;相关产品有：JOSK-M细胞、MCA 38细胞、WM-239-A细胞

BC-021 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁或悬浮，详见产品说明书部分;形态特性：详见产品说明书;相关产品有：Highly Aggressively Proliferating Immortalized细胞、BRL细胞、MDA-MB 453细胞

DHL10 Cells;背景说明：详见相关文献介绍;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：悬浮;形态特性：淋巴母细胞;相关产品有：H1793细胞、TB-1 Lu细胞、32D CL3细胞

TE-14 Cells;背景说明：详见相关文献介绍;传代方法：消化３-５分钟。１：２。３天内可长满。;生长特性：贴壁生长;形态特性：上皮样;相关产品有：A-20细胞、C33A细胞、HEC1A细胞

STBCi314-A Cells(提供STR鉴定图谱)

UWB1.289 Cells;背景说明：详见相关文献介绍;传代方法：１：２传代;生长特性：贴壁生长　;形态特性：详见产品说明书;相关产品有：118MG细胞、NCIH196细胞、FD-LSC-1细胞

TYK-nu Cells;背景说明：详见相关文献介绍;传代方法：１：２传代;生长特性：贴壁生长;形态特性：详见产品说明书;相关产品有：SEG-1细胞、Mv 1 Lu (NBL-7)细胞、HTSMC细胞

OCIAML4 Cells;背景说明：急性髓系白血病；女性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：悬浮;形态特性：详见产品说明书;相关产品有：NG10815细胞、A2780/Taxol细胞、3T3 clone A31细胞

786-0WT Cells;背景说明：该细胞源自一位原发性肾透明细胞癌患者。该细胞有微绒毛和桥粒，能在软琼脂上生长。此细胞生成一种PTH（甲状旁腺素）样的多肽，与乳癌和肺癌中生成的肽相似，其N端序列与PTH相似，具有PTH样活性，分子量为６０００D。;传代方法：１：２传代;生长特性：贴壁生长;形态特性：上皮样;相关产品有：PATU-S细胞、BXPC3细胞、TW 01细胞

MNNG-HOS (Cl#5) Cells;背景说明：骨肉瘤；女性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：P31-FUJ细胞、HCGC细胞、Hi-5细胞

3T3 clone A31 Cells;背景说明：胚胎；成纤维；自发永生；雄性；BALB/c;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：EC-109细胞、NB-4细胞、BDCM细胞

Ly3 Cells;背景说明：弥漫大B淋巴瘤；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：悬浮;形态特性：详见产品说明书;相关产品有：NCI-H295R细胞、MDA-MB-330细胞、LLC-WRC 256细胞

Hu-P-T4 Cells;背景说明：详见相关文献介绍;传代方法：１：２传代;生长特性：贴壁生长;形态特性：详见产品说明书;相关产品有：COV362细胞、697细胞、Menschliche Und Tierische Zellkulture-3细胞

MCF7[MCF-7]人乳腺癌细胞全年复苏|已有STR图谱

H2122 Cells;背景说明：详见相关文献介绍;传代方法：１：３-１：４传代；每周换液２-３次。;生长特性：贴壁生长;形态特性：淋巴母细胞;相关产品有：HTR-8/SV-neo细胞、H2023细胞、RMa-bm细胞

SKRC-20 Cells;背景说明：肾癌;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：NCI-H2342细胞、Line 697细胞、CNE2Z细胞

YES 2 Cells;背景说明：食管鳞癌；男性;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：SNU520细胞、149 PT细胞、aTC1 Clone 6细胞

Madison 109 Cells;背景说明：肺癌；BALB/c;传代方法：１：２-１：３传代；每周换液２-３次。;生长特性：贴壁;形态特性：详见产品说明书;相关产品有：NCI-BL2141细胞、GC9811-P细胞、TSU-Pr1细胞

P31FUJ Cells;背景说明：详见相关文献介绍;传代方法：１：５传代;生长特性：悬浮生长;形态特性：淋巴母细胞;相关产品有：SNU251细胞、MDA435细胞、EOC20细胞

D324 Med Cells;背景说明：详见相关文献介绍;传代方法：１：４-１：６传代；每周换液２-３次。;生长特性：贴壁生长;形态特性：多边形;相关产品有：Vero 76细胞、CL1-0细胞、Ls-174-T细胞

BayGenomics ES cell line CSC010 Cells(提供STR鉴定图谱)

BayGenomics ES cell line RRS529 Cells(提供STR鉴定图谱)

BayGenomics ES cell line YTC010 Cells(提供STR鉴定图谱)

III A4.1 Cells(提供STR鉴定图谱)

PCRP-RFX5-1D11 Cells(提供STR鉴定图谱)

L6.C11 Cells(提供STR鉴定图谱)

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More than apples and oranges -- detecting cancer with a fruit fly's antenna.

Sci. Rep. 4:3576-3576(2014)

PubMed=24456987; DOI=10.1186/1755-8166-7-8; PMCID=PMC3914704

Rondon-Lagos M., Verdun Di Cantogno L., Marchio C., Rangel N., Payan-Gomez C., Gugliotta P., Botta C., Bussolati G., Ramirez-Clavijo S.R., Pasini B., Sapino A.

Differences and homologies of chromosomal alterations within and between breast cancer cell lines: a clustering analysis.

Mol. Cytogenet. 7:8.1-8.14(2014)

PubMed=24618588; DOI=10.1371/journal.pone.0091433; PMCID=PMC3950186

Chernobrovkin A.L., Zubarev R.A.

Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.

PLoS ONE 9:E91433-E91433(2014)

PubMed=24670534; DOI=10.1371/journal.pone.0092047; PMCID=PMC3966786

Varma S., Pommier Y., Sunshine M., Weinstein J.N., Reinhold W.C.

High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.

PLoS ONE 9:E92047-E92047(2014)

PubMed=25321415; DOI=10.1210/me.2014-1229; PMCID=PMC4250366

Li Y., Arao Y., Hall J.M., Burkett S.S., Liu L.-W., Gerrish K., Cavailles V., Korach K.S.

Research resource: STR DNA profile and gene expression comparisons of human BG-1 cells and a BG-1/MCF-7 clonal variant.

Mol. Endocrinol. 28:2072-2081(2014)

PubMed=25960936; DOI=10.4161/21624011.2014.954893; PMCID=PMC4355981

Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.

A catalog of HLA type, HLA expression, and neo-epitope candidates in human cancer cell lines.

OncoImmunology 3:e954893.1-e954893.12(2014)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652

Cowley G.S., Weir B.A., Vazquez F., Tamayo P., Scott J.A., Rusin S., East-Seletsky A., Ali L.D., Gerath W.F.J., Pantel S.E., Lizotte P.H., Jiang G.-Z., Hsiao J., Tsherniak A., Dwinell E., Aoyama S., Okamoto M., Harrington W., Gelfand E.T., Green T.M., Tomko M.J., Gopal S., Wong T.C., Li H.-B., Howell S., Stransky N., Liefeld T., Jang D., Bistline J., Meyers B.H., Armstrong S.A., Anderson K.C., Stegmaier K., Reich M., Pellman D., Boehm J.S., Mesirov J.P., Golub T.R., Root D.E., Hahn W.C.

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.

Sci. Data 1:140035-140035(2014)

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)

PubMed=25807930; DOI=10.1002/anie.201500342; PMCID=PMC4471546

Broncel M., Serwa R.A., Ciepla P., Krause E., Dallman M.J., Magee A.I., Tate E.W.

Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development.

Angew. Chem. Int. Ed. Engl. 54:5948-5951(2015)

PubMed=25828948; DOI=10.1093/jnci/djv073

Lee A.V., Oesterreich S., Davidson N.E.

MCF-7 cells -- changing the course of breast cancer research and care for 45 years.

J. Natl. Cancer Inst. 107:djv073.1-djv073.4(2015)

PubMed=25877200; DOI=10.1038/nature14397

Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.

A resource for cell line authentication, annotation and quality control.

Nature 520:307-311(2015)

PubMed=25892236; DOI=10.1016/j.celrep.2015.03.050; PMCID=PMC4425736

Lawrence R.T., Perez E.M., Hernandez D., Miller C.P., Haas K.M., Irie H.Y., Lee S.-I., Blau C.A., Villen J.

The proteomic landscape of triple-negative breast cancer.

Cell Rep. 11:630-644(2015)

PubMed=26026074

Comsa S., Cimpean A.M., Raica M.

The story of MCF-7 breast cancer cell line: 40 years of experience in research.

Anticancer Res. 35:3147-3154(2015)

PubMed=26055192; DOI=10.1021/acs.jproteome.5b00375

Cifani P., Kirik U., Waldemarson S., James P.

Molecular portrait of breast-cancer-derived cell lines reveals poor similarity with tumors.

J. Proteome Res. 14:2819-2827(2015)

PubMed=26116361; DOI=10.1158/1535-7163.MCT-15-0143

Ribas R., Pancholi S., Guest S.K., Marangoni E., Gao Q., Thuleau A., Simigdala N., Polanska U.M., Campbell H., Rani A., Liccardi G., Johnston S.R.D., Davies B.R., Dowsett M., Martin L.-A.

AKT antagonist AZD5363 influences estrogen receptor function in endocrine-resistant breast cancer and synergizes with fulvestrant (ICI182780) in vivo.

Mol. Cancer Ther. 14:2035-2048(2015)

PubMed=26330541; DOI=10.1074/mcp.M115.050484; PMCID=PMC4638033

Wu X.-Y., Zahari M.S., Renuse S., Nirujogi R.S., Kim M.-S., Manda S.S., Stearns V., Gabrielson E.W., Sukumar S., Pandey A.

Phosphoproteomic analysis identifies focal adhesion kinase 2 (FAK2) as a potential therapeutic target for tamoxifen resistance in breast cancer.

Mol. Cell. Proteomics 14:2887-2900(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878

Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.

TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.

Genome Med. 7:118.1-118.7(2015)

PubMed=27331101; DOI=10.1016/j.dib.2016.05.040; PMCID=PMC4905937

Aumsuwan P., Khan S.I., Khan I.A., Walker L.A., Dasmahapatra A.K.

Gene expression profiling and pathway analysis data in MCF-7 and MDA-MB-231 human breast cancer cell lines"