"MDA-MB-231人乳腺癌细胞代次低|培养基|送STR图谱
传代比例:1:2-1:4(首次传代建议1:2)
生长特性:贴壁生长
【细胞培养经验分享】启蒙老师的重要性:一般进实验室都有师兄师姐带着做,他们就是你做细胞的启蒙老师。他们的操作手法、细节、理论讲解就成了你操作的准则,如营养液、细胞瓶的摆放位置、灭菌处理程序、开盖手法、细胞吹打手法等等。要学会他们的正确操作,在第一次的时候就要重视。像养孩子一样养细胞,细胞有时真的很脆弱,最好每天都去看看它,以防止出现培养箱缺水、缺二氧化碳、停电、温度不够等异常现象,也好及时解决这些意外,避免重复实验带来的更大痛苦。好细胞要及时保种:细胞要分批传代,这样即使有一批出了问题,还有一批备用的。像后者一般人可能不容易做到。但这是我血的教训,有一次细胞污染了,全军覆没。当时可后悔没有保种。细胞跟人一样,不同的细胞,培养特性是不一样的。培养过程中要细细体会,不同细胞系使用不同的培养基和血清。
换液周期:每周2-3次
CP-70 Cells;背景说明:卵巢癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MHCC97-H细胞、SNU-5细胞、UMC-11细胞
MMAc-Serum Free Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:详见产品说明书;相关产品有:OCI-Ly18细胞、TE-4细胞、KTCTL140细胞
CCRFCEM Cells;背景说明:G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。此细胞系从香港收集而来。;传代方法:1:2传代。3天内可长满。;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:MM-1S细胞、SR786细胞、HTori:3细胞
MDA-MB-231人乳腺癌细胞代次低|培养基|送STR图谱
背景信息:-MB-231来自患有转移乳腺腺癌的51岁女病人的胸水。在裸鼠和ALS处理的BALB/c小鼠中,它能形成低分化腺癌(III级)。
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DSMZ菌株保藏中心成立于1969年,是德国的国家菌种保藏中心。该中心一直致力于细菌、真菌、质粒、抗菌素、人体和动物细胞、植物病毒等的分类、鉴定和保藏工作。DSMZ菌种保藏中心是欧洲规模最大的生物资源中心,保藏有动物细胞500多株。Riken BRC成立于1920年,是英国的国家菌种保藏中心。该中心一直致力于细菌、真菌、植物病毒等的分类、鉴定和保藏工作。日本Riken BRC(Riken生物资源保藏中心)是全球三大典型培养物收集中心之一。Riken保藏中心提供了很多细胞系。在世界范围内,这些细胞系,都在医学、科学和兽医中具有重要意义。Riken生物资源中心支持了各种学术、健康、食品和兽医机构的研究工作,并在世界各地不同组织的微生物实验室和研究机构中使用。
产品包装:复苏发货:T25培养瓶(一瓶)或冻存发货:1ml冻存管(两支)
来源说明:细胞主要来源ATCC、ECACC、DSMZ、RIKEN等细胞库
MDA-MB-231人乳腺癌细胞代次低|培养基|送STR图谱
物种来源:人源、鼠源等其它物种来源
EFM-19 Cells;背景说明:乳腺管癌;胸腔积液转移;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:SKMES细胞、RD-ES细胞、HT-115细胞
AZ-521 Cells;背景说明:详见相关文献介绍;传代方法:1:4传代;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:上皮样;相关产品有:MADISON LUNG TA-109细胞、MOLT.4细胞、T98-G细胞
HCC9724 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:BIU87细胞、MRAEC细胞、T-47D细胞
Hs729 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:Panc 08.13细胞、HONE1细胞、MC3T3-E细胞
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形态特性:上皮细胞样
细胞冻存复苏材料与方法步骤:常用的细胞冷冻贮存器为贮存器,规格有35L和50L两种。使用时要注意以下几点:(1)一般两周需充一次,至少一个月充一次。温度达-196℃,使用时注意勿让溅到皮肤上,以免引起冻伤。(2)容器为双层结构,中间为真空层,瓶口有双层焊接处,应防止焊接部裂开。(3)在装入时,要注意缓慢小心,并用厚纸卷筒或制漏斗作引导,使直达瓶底,如有专用灌注装置则更HAO。若为初次使用,加时更要缓慢,以免温度骤降而使容器损坏。细胞冻存时常备的材料有:0.25%胰蛋白酶,含10%~20%的血清培养,DMSO(分析纯)或无色新鲜甘油(121°C蒸气GAO压消毒),2mL安瓿(或专用细胞冻存管)、吸管、离心管、喷灯、纱布袋(或冻存管架)等。主要操作步骤为:(1)选择处于对数生长期的细胞,在冻存前一天ZuiHAO换。将多个培养瓶中的细胞培养 去掉,用0.25%胰蛋白酶消化。适时去掉胰蛋白酶,加入少量新培养。用吸管吸取培养反复吹打瓶壁上的细胞,使其成为均匀分散的细胞悬。悬浮生产细胞则不要消化处理。然后将细胞收集于离心管中离心(1000r/min,10分钟)。(2)去上清,加入含20%小牛血清的完全培养基,于4℃预冷15分钟后,逐滴加入已无菌的DMSO或甘油,用吸管轻轻吹打使细胞均匀,细胞浓度为3×106~1×107/mL之间。(3)将上述细胞分装于安瓿或专用冷冻塑料管中,安瓿装1~1.5mL在火焰喷灯上封口,封口处要完全封闭,圆滑无勾。冷冻管要将盖子盖紧,并标记HAO细胞名称和冻存日期,同时作HAO登记(日期、细胞种类及代次、冻存支数)。(4)将装HAO细胞的安瓿或冻存管装入沙布袋内;置于容器颈口处存放过夜,次日转入中。采用控制降温速度的方法也可采用下列步骤:先将安瓿置入4℃冰箱中2~3小时,再移至冰箱冷冻室内3~4小时,再吊入容器颈气态部分存放2小时,Zui后沉入中。细胞冻存在中可以长期保存,但为妥善起见,冻存半年后,ZuiHAO取出一只安瓿细胞复苏培养,观察生长情况,然后再继续冻存。
NCI-H441 Cells;背景说明:NCI-H441建系于1982年(A.F.Gazdar,etal.)。该细胞分离自一名肺腺癌患者的心包液。该细胞能在半固体琼脂糖中形成克隆,并能表达肺泡表面活性蛋白A。该细胞在有血清培养基中倍增时间为58小时,在无血清培养基中倍增时间为99-138小时。;传代方法:1:3传代,2-3天传一代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:HCC-366细胞、MN 60细胞、DITNC1细胞
UMUC14 Cells;背景说明:肾癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Mono Mac 1细胞、CT26-clone 25细胞、MNNG-HOS细胞
EoL-1 cell Cells;背景说明:急性髓系白血病;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:Hs746-T细胞、NGP细胞、143BTK-细胞
F-36P Cells;背景说明:详见相关文献介绍;传代方法:每周2次换液;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:Hepa 1-6细胞、NB9细胞、PLMVEC细胞
DF1 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:成纤维母细胞样;相关产品有:MD Anderson-Metastatic Breast-468细胞、H838细胞、MC3T3-E1 Subclone 14细胞
HEC1A Cells;背景说明:这株细胞及其亚株HEC-1-B是H.Kuramoto及其同事1968年从一位IA期子宫内膜癌患者身上分离得到的。PAF可以诱导其c-fos的表达。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:NCIH2029细胞、GES1细胞、Bac12F5细胞
118 MG Cells;背景说明:注意: 据报道来自不同个体的胶质母细胞瘤细胞株U-118 MG (HTB-15) 和 U-138 MG (HTB-16)有着一致的VNTR和相近的STR模式。 U-118 MG 和 U-138 MG细胞遗传学上很相似并有至少六个衍生标记染色体。 这是1966年至1969年间J. Ponten和同事从恶性神经胶质瘤中构建的细胞株中的一株(其它包括ATCC HTB-14和 ATCC HTB-16 and ATCC HTB-17)。 1987年用BM-Cycline培养6周去除了支原体污染。 ;传代方法: 消化3-5分钟。1:2传代。3天内可长满。;生长特性:贴壁生长;形态特性:混合型;相关产品有:HNE3细胞、LK-2细胞、B16/F10细胞
IHH4 Cells;背景说明:甲状腺乳头状癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:NCTC929细胞、NCI-H1836细胞、VP267细胞
THPI Cells;背景说明:该细胞从一名1岁的患有急性单核细胞性白血病的男孩的外周血中分离建立。该细胞可以吞噬乳胶颗粒和激活的红细胞,细胞膜和胞浆内均没有免疫球蛋白,表达C3R和FcR;可受佛波酯TPA诱导向单核系方向分化;可作为转染宿主。;传代方法:维持细胞浓度在2-4×105-8×105/ml,勿超过1×106/ml;2-3天换液1次。;生长特性:悬浮生长;形态特性:单核细胞;相关产品有:SK-N-BE(2C)细胞、NBL-12细胞、HS940细胞
HCC0202 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:B-CPAP细胞、HT-29/CX-1细胞、OV-1063细胞
Psi2-DAP Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:Ca759细胞、UACC812细胞、TC-1[JHU-1]细胞
MAVER Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:5传代;2-3天换液1次。;生长特性:悬浮生长;形态特性:淋巴母细胞;相关产品有:HuH 6细胞、FL-83B细胞、P3.NS-1/1.Ag4.1细胞
Human Corneal Endothelial Cells-12 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:SGC-7901/DDP细胞、BAEC细胞、UPCI-SCC-154细胞
DMS153 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:PL-9细胞、NCI-H1819细胞、SK-Mel1细胞
UMNSAH-DF-1 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:成纤维母细胞样;相关产品有:U343MG细胞、WM115mel细胞、VAESBJ细胞
H250 Cells;背景说明:小细胞肺癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:半贴壁;形态特性:详见产品说明书;相关产品有:VM-CUB1细胞、RERF-LC-MS细胞、NU-GC-2细胞
alpha TC1.6 Cells;背景说明:胰岛素瘤;a细胞;C57BL/6xDBA/2;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HO1N1细胞、MIN-6细胞、BC-024细胞
12 079 Cells(提供STR鉴定图谱)
Abcam HeLa BIRC2 KO Cells(提供STR鉴定图谱)
AG14953 Cells(提供STR鉴定图谱)
BayGenomics ES cell line RRE078 Cells(提供STR鉴定图谱)
BayGenomics ES cell line XG014 Cells(提供STR鉴定图谱)
BY00861 Cells(提供STR鉴定图谱)
CU-28 Cells(提供STR鉴定图谱)
DA05490 Cells(提供STR鉴定图谱)
Genomeditech Jurkat H_FcgammaRIIIa Reporter Cells(提供STR鉴定图谱)
Walker-256 Cells;背景说明:乳腺癌;雌性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:AR4-IP细胞、HCC0366细胞、HEL-92_1_7细胞
MDA-MB-231人乳腺癌细胞代次低|培养基|送STR图谱
HSC4 Cells;背景说明:详见相关文献介绍;传代方法:1 x 10^5 cells/10cm dish;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:H1105细胞、mIMCD-3细胞、NSH细胞
HIBEpiC Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:HEK 293A细胞、Normal Rat Kidney-52E细胞、H2107细胞
HEC-1-A Cells;背景说明:这株细胞及其亚株HEC-1-B是H.Kuramoto及其同事1968年从一位IA期子宫内膜癌患者身上分离得到的。PAF可以诱导其c-fos的表达。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:WSU-DLCL2细胞、U-87 MG细胞、Ramos.2G6.4C10细胞
SW-1573 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:MDBK (NBL-1)细胞、IM 9细胞、NR 8383细胞
NCIADR.RES Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:SUNE 1细胞、SU-DHL-5细胞、3LL细胞
1F10 [Mouse hybridoma against pig IgA] Cells(提供STR鉴定图谱)
7404 Cells;背景说明:用Northernblot方法,未能检测到细胞中1.3kbLFIRE-1/HFREP-1mRNA的表达。;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:4-1st细胞、Hs 706.T细胞、Hs 821.T细胞
McCoy Cells;背景说明:成纤维 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:NCIH1373细胞、HAVSMC细胞、CA-46细胞
NOR-10 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:ROS-17/2.8细胞、H146细胞、SKNFI细胞
NuTu 19 Cells;背景说明:卵巢癌;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:RGE细胞、PT-K75细胞、HB611细胞
FaDu Cells;背景说明:1968年从一位印度下咽骨肿瘤患者的钻孔活组织切片中建立了FaDu细胞株。在建立的细胞株中发现细胞质中含有成束的细丝,并且细胞分界上的桥粒特别突出。;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:Farage细胞、D-407细胞、SK-MES-1细胞
KU.812 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代。3天内可长满;生长特性:悬浮生长;形态特性:骨髓母细胞;相关产品有:J-774A.1细胞、Hi-five细胞、HEK-293-H细胞
KU.812 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代。3天内可长满;生长特性:悬浮生长;形态特性:骨髓母细胞;相关产品有:J-774A.1细胞、Hi-five细胞、HEK-293-H细胞
TPC-1 Cells;背景说明:甲状腺乳头状癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:OUMS23细胞、D407 RPE细胞、RTE细胞
Hair iPS1 Cells(提供STR鉴定图谱)
HAP1 RRAGA (-) 2 Cells(提供STR鉴定图谱)
PLC PRF 5 Cells;背景说明:该细胞系分泌乙肝病毒表面抗原(HBsAg)。 此细胞系原先被支原体污染,后用BM-cycline去除支原体;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:RLE-6TN细胞、U-2932细胞、DMS-53细胞
SMMC 7721 Cells;背景说明:用Northernblot方法,未能检测到细胞中1.3kbLFIRE-1/HFREP-1mRNA的表达。;传代方法:1:3传代,2-3天换液一次;生长特性:贴壁生长;形态特性:上皮样;相关产品有:EBNA293细胞、NCIH1792细胞、H2347细胞
MOLP-8 Cells;背景说明:浆细胞骨髓瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:Hs 742.T细胞、HSC-4细胞、SNU475细胞
SK.MEL.2 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:6传代,2-3天换液1次。;生长特性:贴壁生长;形态特性:多边形的;相关产品有:Strain KB细胞、NIH-3T3-L1细胞、95-D细胞
SCLC-21H Cells;背景说明:小细胞肺癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:SPCA-1细胞、NCIH2107细胞、231-luc细胞
MDAMB175 Cells;背景说明:该细胞源自一位54岁患有乳腺导管癌白人女性的胸腔积液。;传代方法:1:2—1:6传代,每周换液2—3次;生长特性:松散贴壁生长;形态特性:上皮细胞样;相关产品有:FCCH1018细胞、Reuber H-35细胞、H292细胞
MA-104 Cells;背景说明:胚肾;自发永生;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Rin-M-5F细胞、RT4-D6P2T细胞、UMR106细胞
HEK293-EBNA1 Cells;背景说明:详见相关文献介绍;传代方法:1:4-1:10传代;每周2次。;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:16HBE细胞、M21细胞、IPECJ2细胞
HyCyte AC16 hRYR2_p.D179E_c.537T>G Cells(提供STR鉴定图谱)
LB-MEL-1 Cells(提供STR鉴定图谱)
MWF Cells(提供STR鉴定图谱)
OU-LC-KM Cells(提供STR鉴定图谱)
RPMI-7262 Cells(提供STR鉴定图谱)
Ubigene HeLa VEGFC KO Cells(提供STR鉴定图谱)
XPHF1WI LCL Cells(提供STR鉴定图谱)
HG02800 Cells(提供STR鉴定图谱)
HCC-1500 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每3-5天换液;生长特性:贴壁生长;形态特性:上皮样;相关产品有:SKMES1细胞、Nb-2细胞、SW-1783细胞
SNT-8 Cells;背景说明:NK/T细胞淋巴瘤;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:Vero E-6细胞、HPAEC细胞、HET-1A细胞
NCIH2009 Cells;背景说明:详见相关文献介绍;传代方法:每周换液2-3次。;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:FD-LSC-1细胞、CCD1095Sk细胞、CT26.WT细胞
Hs839T Cells;背景说明:详见相关文献介绍;传代方法:1:2传代,2-3天换液1次。;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:U-87MG ATCC细胞、PANC0203细胞、SK-Hep1细胞
F98 Cells;背景说明:恶性胶质瘤;雌性;Fischer大鼠;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MC-CAR细胞、YES 2细胞、YAC-1细胞
F98 Cells;背景说明:恶性胶质瘤;雌性;Fischer大鼠;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:MC-CAR细胞、YES 2细胞、YAC-1细胞
SK BR 03 Cells;背景说明:这株细胞源自胸水。没有病毒颗粒。亚显微结构特征包括微丝和桥粒,肝糖原颗粒,大溶酶体,成束的细胞质纤丝。SK-BR-3细胞株过表达HER2/c-erb-2基因产物。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:3T3NIH细胞、KTC1细胞、Hs 739.T细胞
AR-42J Cells;背景说明:在肾上腺皮质激素刺激下可诱导出外分泌活性,并伴有广泛的内质网重构。;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:SCC4细胞、RAEC细胞、RCC_7860细胞
253J-Bladder-V Cells;背景说明:膀胱癌;淋巴结转移;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:LA 4细胞、KMST-6细胞、PAI细胞
FRO81-2 Cells;背景说明:未分化甲状腺癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:RIN-m细胞、SW1088细胞、NCI-H196细胞
F442A Cells;背景说明:脂肪前体细胞;雄性;Swiss albino;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:TO 175.T细胞、H23细胞、SN12-PM6细胞
Factor Dependent Cell-Paterson 1 Cells;背景说明:详见相关文献介绍;传代方法:2-3天换液1次;生长特性:悬浮生长;形态特性:淋巴母细胞;相关产品有:HeLa S-3细胞、RPMI-8402细胞、G-Olig2细胞
rRTEC Cells;背景说明:肾小管;上皮 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:H596细胞、NCI-H1105细胞、MCF7ADR细胞
HD-LM-2 Cells;背景说明:霍奇金淋巴瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:EOC 20细胞、GM04679细胞、FU-MMT-1细胞
E.L.4 Cells;背景说明:EL4是从用9,10-二甲基-1,2-并蒽在C57BL小鼠中诱导的淋巴瘤中建立的。 能抗0.1 mM 化可的松,对20 mcg/ml PHA敏感。 还有一个亚株(EL4.IL-2, ATCC TIB-181)可以生成高水平的IL-2。 检测表明肢骨发育畸形病毒(鼠痘)阴性。;传代方法:1:2传代;生长特性:悬浮生长;形态特性:详见产品说明书;相关产品有:HEL9217细胞、HEL299细胞、SVHUC细胞
TBM-54 Cells(提供STR鉴定图谱)
TE10 Cells;背景说明:详见相关文献介绍;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮样;相关产品有:V-79-4细胞、GM12878细胞、MPC5细胞
B10BR Cells;背景说明:永生化黑素 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:NCI-H838细胞、OVCA5细胞、IPEC-1细胞
H-2087 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:4传代;每周换液2次。;生长特性:悬浮生长,有少数细胞疏松贴壁;形态特性:上皮样;相关产品有:PL-12细胞、NIH/3T3细胞、MDA-MB-453细胞
OVCA8 Cells;背景说明:卵巢癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:GM03320细胞、RCC-10细胞、hA549细胞
KBM-7 Cells;背景说明:慢性髓原白血病;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:KY-50细胞、GS-9L细胞、SK-N-MC细胞
NCI-H735 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:OVCA433细胞、MES-SA Dx5细胞、OsACL细胞
MDA-MB-231人乳腺癌细胞代次低|培养基|送STR图谱
IGROV 1 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:HDF-a细胞、NCI-Hut 125细胞、OLN-93细胞
GM12878 Cells;背景说明:B淋巴细胞;EBV转化;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:KM-12细胞、COR-L26细胞、HDLM2细胞
Farage OL Cells;背景说明:Farage细胞源于一名患有弥漫性大B细胞淋巴瘤(DLBCL)白人女性的活检淋巴组织,由HBen-Bassat建系。经IL-4处理,该细胞CD21,CD22,CD54和CD58表达上调,而CD21,CD22,andCD38表达下调。;传代方法:1:3传代,2-3天传一代;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:F-36P细胞、SK-RC 52细胞、SK-MEL5细胞
PTK2 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:JIII细胞、293-GP细胞、H-2444细胞
University of Michigan-Urothelial Carcinoma-14 Cells;背景说明:肾癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Fortner's melanotic melanoma #3细胞、OVSAHO细胞、PL45细胞
UCLA NPA-87-1 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:SNU-16细胞、Gejiu Lung Carcinoma-82细胞、NCIH146细胞
C6 Cells;背景说明:胶质细胞株C6是由Benda等用N-nitrosomethylurea诱导的大鼠胶质瘤克隆,并经过一系列的体外培养和动物传代交替后建成的。 当细胞从低密度生长到满瓶时,S-100产量增加10倍。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:L-02细胞、NCI-H2444细胞、LS-180细胞
SR786 Cells;背景说明:间变性大细胞淋巴瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:UCW 100细胞、HEH2细胞、P3X63细胞
BayGenomics ES cell line CSI758 Cells(提供STR鉴定图谱)
BayGenomics ES cell line SYA102 Cells(提供STR鉴定图谱)
CB-F7 Cells(提供STR鉴定图谱)
Ltk-8-30-84 Cells(提供STR鉴定图谱)
RAW 264.7 Rab8a KO Cells(提供STR鉴定图谱)
RN33B.LV-AA2 Cells(提供STR鉴定图谱)
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