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网站主页 化工产品目录 生物 细胞培养 细胞系 人细胞系 HCT 116 HCT 116人结肠癌细胞代次低|培养基|送STR图谱
  • HCT 116人结肠癌细胞代次低|培养基|送STR图谱
  • HCT 116人结肠癌细胞代次低|培养基|送STR图谱
  • HCT 116人结肠癌细胞代次低|培养基|送STR图谱

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HCT 116人结肠癌细胞代次低|培养基|送STR图谱

HCT 116
询价 1000000Cells/瓶 起订
2000000Cells/瓶 起订
上海 更新日期:2025-02-23

上海宾穗生物科技有限公司

VIP1年
联系人:刘经理
手机:13641930791 拨打
邮箱:3180807324@qq.com

产品详情:

中文名称:
HCT 116人结肠癌细胞代次低|培养基|送STR图谱
英文名称:
HCT 116
品牌:
ATCC、DSMZ等
产地:
美国、欧洲、德国等
保存条件:
低温避光
纯度规格:
HCT 116人结肠癌细胞代次低|培养基|送STR图谱
产品类别:
ATCC细胞库
种属:
详见细胞说明书
组织:
详见细胞说明书
细胞系:
详见细胞说明书
细胞形态:
详见细胞说明书
生长状态:
详见细胞说明书
靶点:
详见细胞说明书
应用:
详见细胞说明书
货号:
详见细胞说明书
规格:
1*10^6cells/T25(1瓶)或1ml冻存管(2支)
是否进口:
来源ATCC、DSMZ、ECACC等细胞库
组织来源:
详见细胞说明书
是否是肿瘤细胞:
详见细胞说明书
器官来源:
详见细胞说明书
品系:
详见细胞说明书
免疫类型:
详见细胞说明书
物种来源:
人源或其它动物来源等
保质期:
可长期保存(液氮低温冻存)

"HCT 116人结肠癌细胞代次低|培养基|送STR图谱

传代比例:1:2-1:4(首次传代建议1:2)

生长特性:贴壁生长

细胞系的选择需要考虑到细胞系的功能特点、生长速率、铺板效率、生长条件和生长特征、克隆效率、培养方式等因素,如果您想高产量表达重组蛋白,您可以选择可以悬浮生长的快速生长细胞系。细胞培养的操作步骤主要包括传代、换液、冻存和复苏。这些步骤确保了细胞能够在实验室环境中长期存活并继续增殖。传代是将细胞从一个容器转移到另一个容器的过程,以扩大细胞数量;换液是为了清除代谢废物并补充新鲜培养基;冻存则是为了长期保存细胞,而复苏则是重新激活冷冻保存的细胞使其恢复正常生长。

换液周期:每周2-3次

Jurkat (clone E6-1) Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:SW756细胞、CEM-CCRF (CAMR)细胞、MOLT-16细胞

Stanford University-Diffuse Histiocytic Lymphoma-10 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:淋巴母细胞;相关产品有:NHEK细胞、T-HEECs细胞、SV40-MES13细胞

EnCa1 Cells;背景说明:内膜腺癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:K7M2-WT细胞、CHL-11细胞、NCI-H2023细胞

HCT 116人结肠癌细胞代次低|培养基|送STR图谱

背景信息:是一种人结肠癌细胞系,是由M·Brattain等人于1979年从患结肠癌的男性病人中分离的三株恶性细胞中的一株。HCT 116细胞在半固体琼脂糖培养基中形成克隆;HCT 116细胞在无胸腺裸鼠有致瘤性,形成肿瘤结节。

┈订┈购(技术服务)┈热┈线:1┈3┈6┈4┈1┈9┈3┈0┈7┈9┈1【微信同号】┈Q┈Q:3┈1┈8┈0┈8┈0┈7┈3┈2┈4;

DSMZ菌株保藏中心成立于1969年,是德国的国家菌种保藏中心。该中心一直致力于细菌、真菌、质粒、抗菌素、人体和动物细胞、植物病毒等的分类、鉴定和保藏工作。DSMZ菌种保藏中心是欧洲规模最大的生物资源中心,保藏有动物细胞500多株。Riken BRC成立于1920年,是英国的国家菌种保藏中心。该中心一直致力于细菌、真菌、植物病毒等的分类、鉴定和保藏工作。日本Riken BRC(Riken生物资源保藏中心)是全球三大典型培养物收集中心之一。Riken保藏中心提供了很多细胞系。在世界范围内,这些细胞系,都在医学、科学和兽医中具有重要意义。Riken生物资源中心支持了各种学术、健康、食品和兽医机构的研究工作,并在世界各地不同组织的微生物实验室和研究机构中使用。

产品包装:复苏发货:T25培养瓶(一瓶)或冻存发货:1ml冻存管(两支)

来源说明:细胞主要来源ATCC、ECACC、DSMZ、RIKEN等细胞库

HCT 116人结肠癌细胞代次低|培养基|送STR图谱

物种来源:人源、鼠源等其它物种来源

SNU-5 Cells;背景说明:该细胞来源于一名低分化胃癌患者的转移性腹水,1987年分离建立。该细胞表达CEA和TAG-72。;传代方法:2-3天补液一次。;生长特性:多细胞聚集、悬浮生长;形态特性:上皮细胞样;相关产品有:SKLU01细胞、HCT_116细胞、KYSE-50细胞

MC 3T3-E1 Cells;背景说明:该细胞有多个亚克隆,可以作为体外研究成骨细胞分化的良好模型,尤其是ECM信号通路的作用。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:成纤维细胞样;相关产品有:SUNE1细胞、WBF344细胞、OE19细胞

PC-9/S1 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:SU86-86细胞、H-498细胞、D407细胞

SKG3A Cells;背景说明:详见相关文献介绍;传代方法:2x10^4 cells/ml;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:IOSE29细胞、B-3细胞、SW-48细胞

┈订┈购(技术服务)┈热┈线:1┈3┈6┈4┈1┈9┈3┈0┈7┈9┈1【微信同号】┈Q┈Q:3┈1┈8┈0┈8┈0┈7┈3┈2┈4;

形态特性:上皮细胞样

正确的细胞复苏需知事项:细胞冻存HAO了,接下来要注意什么问题呢?没错,就是记得到时间了,拿出来复苏。那么,细胞复苏的过程中又有哪些该注意的事项呢?细胞活力和形态检查的作用何在?活力检查——千万不要使用不健康的细胞,可能有污染(真菌、支原体等),如果发现有污染毫不犹豫的丢弃!形态检查——检查细胞的固有形态和生长行为。冻存细胞:补充新的培养——在您开始冻存细胞的前一天补充新的培养。在细胞长至70%单层时收获细胞,计数活细胞数,用冻存调整细胞密度~5 x106  s/ml (根据不同的细胞类型调整);冻存——用冻存洗细胞并用冻存重悬细胞,有不同类型的冻存,根据细胞类型选择Zui合适的冻存(常用的冻存成分有):5-10% DMSO——注意确保DMSO不含有其他的毒性物质;5-15%甘油;如果细胞在无血清培养基内生长,应在50%条件培养基内(细胞在无血清培养基内生长24小时)内冻存和复苏。在冻存管上标记HAO细胞类型,日期,冻存人等信息,并保证每冻存管不超过1.5ml。放入罐之前记录冻存管的数量和位置。以Zui快的速度转移冻存管知罐内,因此,此步骤ZuiHAO使用干冰,或者把冻存管浸入装有的小盒内。此外还要注意,在冻存管上没有足够的空间记录细胞的详细信息,做HAO记录是非常非常重要的!还有一个Zui重要的,一定要在异地的罐内保存同样的一份细胞,以免其中的一个罐出现问题!细胞正确的复苏方式和正确的冻存方式同样重要,熟记以下要点:当从罐内取出细胞时,有可能会出现冻存管破裂的情况,使用保护面罩和防护服十分必要;其实,细胞复苏只是一个简单的实验,不过这其中却不可避免有一些需要注意的细节,不然,也不一定会尽如人意。例如说,人身健康方面:一定要记得做HAO防冻工作,戴上护目镜;尽量降低DMSO对细胞的损伤等等。

OVCAR 5 Cells;背景说明:卵巢癌;腹水转移;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Nb-2细胞、MTC-TT细胞、SW527细胞

SCC 15 Cells;背景说明:详见相关文献介绍;传代方法:1:4-1:8传代,2-3天换液1次。;生长特性:贴壁生长;形态特性:详见产品说明书;相关产品有:VeroC1008细胞、NCI-H522细胞、MV4-11细胞

Hs611T Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;每周换液2-3次。;生长特性:混合型;形态特性:淋巴母细胞样;相关产品有:B16-F0细胞、MFD-1细胞、NCIH1385细胞

Strain V Cells;背景说明:肺;自发永生;雄性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:NeHepLxHT细胞、Stanford University-Diffuse Histiocytic Lymphoma-4细胞、SUDHL6细胞

GT38 Cells;背景说明:胃癌;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:TOG细胞、CoCL3细胞、Mv 1 Lu细胞

SUM190 Cells;背景说明:乳腺癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:SW 780细胞、JURKAT E-61细胞、SCC15细胞

8226/S Cells;背景说明:来源于一位61岁的男性浆细胞瘤患者;可产生免疫球蛋白轻链,未检测到重链。;传代方法:按1:2传代,5-6小时可以看到细胞分裂;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:LCD细胞、HCC-4006细胞、293细胞

GM346 Cells;背景说明:皮下结缔组织;自发永生;雄性;C3H/An;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:LN 229细胞、HS-766-T细胞、NCI-H2286细胞

SK-MEL-28 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:8传代,2-3天换液1次。;生长特性:贴壁生长;形态特性:星形的;相关产品有:HSF细胞、AQ-Mel细胞、4-1st细胞

PGBE1 Cells;背景说明:肺巨细胞癌;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Simpson细胞、Clone 15 HL-60细胞、C6661细胞

Mo 59J Cells;背景说明:详见相关文献介绍;传代方法:1:6-1:8传代;每周换液2-3次。;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:GA-10 clone 4细胞、HuTu 80细胞、Ca759细胞

SKNBE(2c) Cells;背景说明:神经母细胞瘤;骨髓转移;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Melan-a细胞、HCA 7细胞、NCI-H522细胞

ChaGo-K-1 Cells;背景说明:详见相关文献介绍;传代方法:1:4-1:8传代;每周换液2次。;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:Michigan Cancer Foundation-7细胞、LS123细胞、CAKI1细胞

TE-32 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:4传代,3-4天换液1次。;生长特性:贴壁生长;形态特性:梭型和大的多核细胞;相关产品有:P3-Jiyoye细胞、hTERT-RPE细胞、EVSAT细胞

HEK-293-F Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;悬浮生长;形态特性:上皮细胞样;相关产品有:201T细胞、NCI-H1048细胞、STO细胞

TC-1[JHU-1] Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长 ;形态特性:详见产品说明书;相关产品有:OCI/AML5细胞、BEL/FU细胞、MLA 144细胞

7404 Cells;背景说明:用Northernblot方法,未能检测到细胞中1.3kbLFIRE-1/HFREP-1mRNA的表达。;传代方法:消化3-5分钟。1:2。3天内可长满。;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:4-1st细胞、Hs 706.T细胞、Hs 821.T细胞

786-O PBRM1 KO 3 Cells(提供STR鉴定图谱)

Abcam MCF-7 EIF2AK3 KO Cells(提供STR鉴定图谱)

AT181TO Cells(提供STR鉴定图谱)

BayGenomics ES cell line RRN227 Cells(提供STR鉴定图谱)

BayGenomics ES cell line YHA296 Cells(提供STR鉴定图谱)

CC22 Cells(提供STR鉴定图谱)

DA00940 Cells(提供STR鉴定图谱)

F4/B8 Cells(提供STR鉴定图谱)

GM07553 Cells(提供STR鉴定图谱)

HCT15 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:P-3J细胞、HuT 102细胞、OVCAR 8细胞

HCT 116人结肠癌细胞代次低|培养基|送STR图谱

NRK52E Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:HCC-1500细胞、SK-MEL-2细胞、SKNEP1细胞

OVCAR3 Cells;背景说明:该细胞1982年由T.C. Hamilton等建系,源自一位60卵巢腺癌的腹水,是卵巢癌抗药性研究的模型。;传代方法:1:2—1:4传代,每周换液2—3次;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:BLO-11细胞、SNB.19细胞、NCI-H711细胞

SW954 Cells;背景说明:详见相关文献介绍;传代方法:1:3-1:6传代,2-3天换液1次。;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:KNS81细胞、MIA-Pa-Ca-2细胞、HCC15细胞

LO2 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:IOSE 29细胞、NR 8383细胞、Hs 695T细胞

Hs 840.T Cells;背景说明:详见相关文献介绍;传代方法:1:4—1:8传代,每周换液2—3次;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:SUIT-2细胞、HCC-2279细胞、Human Liver-7702细胞

B10R Cells(提供STR鉴定图谱)

SNB-19 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;生长特性:贴壁生长 ;形态特性:详见产品说明书;相关产品有:LP1细胞、CCD18细胞、SKOV3细胞

TPC1 Cells;背景说明:甲状腺乳头状癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:TE-4细胞、Mono Mac 1细胞、U-373MG细胞

RCF Cells;背景说明:心肌;成纤维 Cells;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HA细胞、Malme-3 M细胞、MRASMC细胞

AN-3 Cells;背景说明:AN3CA细胞建系于1964年。它衍生于子宫内膜癌患者淋巴结转移组织,具有癌细胞的基本特性,能在体外长期传代培养,接种实验动物产生明显肿瘤。但细胞的生物学特性及超微结构尚未深入研究,仅发现该细胞系促黑激素合成为阴性。细胞常用于人子宫内膜癌细胞生物学及其相关特性研究。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:U373MG细胞、COLO 320细胞、HCM细胞

K299 Cells;背景说明:间变性大细胞淋巴瘤;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:CL34细胞、H-2347细胞、NK-92细胞

CCD 841 CoTr Cells;背景说明:结肠癌;SV40转化;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:COLO-320HSR细胞、CHP212细胞、C3H-10T1/2细胞

AU-565 Cells;背景说明:详见相关文献介绍;传代方法:1:4—1:6传代;每3-5天换一次液。;生长特性:贴壁生长;形态特性:上皮细胞;相关产品有:LCLC-103H细胞、Clone 166细胞、Panc04.03细胞

T47D Cells;背景说明:浸润性导管癌;胸腔积液转移;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:AR-42J细胞、Hs895T细胞、Hs888T细胞

GM28877 Cells(提供STR鉴定图谱)

HAP1 PEX3 (-) 1 Cells(提供STR鉴定图谱)

JM-Jurkat Cells;背景说明:该细胞源自一位14岁患有T淋巴细胞白血病男性的外周血;传代方法:保持细胞密度在3—9×105cells/ml之间,1:5—1:10传代,每周换液2—3次;生长特性:悬浮生长;形态特性:圆形,单个或呈片;相关产品有:PFSK-1细胞、TE85细胞、Hs274T细胞

P3HR-1 Cells;背景说明:详见相关文献介绍;传代方法:每2-3天换液;生长特性:悬浮生长 ;形态特性:淋巴母细胞样;相关产品有:CA-OV-3细胞、REC 1细胞、KMS11细胞

MDAMB468 Cells;背景说明:该细胞是1977年由CailleauR等从一位患有转移性乳腺癌的51岁黑人女性的胸腔积液中分离得到的。虽然供体组织的G6PD等位基因杂合,但此细胞株始终表现为G6PDA表型。P53基因273位密码子存在G→A突变,从而导致Arg→His替代。每个细胞上存在1×106个EGF受体。;传代方法:1:2-1:4传代;2-3天换液1次;生长特性:贴壁生长;形态特性:上皮样;相关产品有:HT1080细胞、BC-023细胞、IM9细胞

KYSE0030 Cells;背景说明:来源于一位64岁,患有高分化的中段食管鳞癌的男性患者。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:上皮细胞样;相关产品有:hOMF细胞、P31/Fujioka细胞、Human Epithelioma-2细胞

Hs 683 Cells;背景说明:该细胞源自76岁白人男性的左颞叶侧胶质瘤组织,有微绒毛,无桥粒。 ;传代方法:1:4传代,每周换液2次;生长特性:贴壁生长;形态特性:成纤维细胞;相关产品有:RDES细胞、Hs606细胞、HuH-6细胞

HEC1-A Cells;背景说明:这株细胞及其亚株HEC-1-B是H.Kuramoto及其同事1968年从一位IA期子宫内膜癌患者身上分离得到的。PAF可以诱导其c-fos的表达。;传代方法:消化3-5分钟,1:2,3天内可长满;生长特性:贴壁生长;形态特性:上皮样;相关产品有:C33A细胞、hA549细胞、LAPC-4细胞

NCTC-1469 Cells;背景说明:1952年建系,源于正常C3H/An小鼠的肝脏组织,表达H-2K抗原,鼠痘病毒阴性。;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:淋巴母细胞;相关产品有:COLO-357细胞、Jeko1细胞、FRhK4细胞

LS174 Cells;背景说明:LS 174T是LS 180 (ATCC CL 187)结肠腺癌细胞株的胰蛋白酶化变种。 它比亲本更易传代,象LS 180一样生成大量的癌胚抗原(CEA)。 电镜研究表明有丰富的微丝和细胞质粘液素液泡。 直肠抗原3阳性。 p53抗原表达阴性,但mRNA表达阳性。 与ATCC CL-187来源于同一个肿瘤。LS 174T细胞角蛋白染色阳性。 癌基因c-myc, N-myc, H-ras, N-ras, Myb, 和 fos的表达呈阳性。 癌基因k-ras和sis的表达未做检测。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:HIBEC细胞、33604细胞、H1838细胞

HSAEC49-KT Cells(提供STR鉴定图谱)

KOLF2.1J SERPINI1 4.6KBDEL DEL/DEL Cells(提供STR鉴定图谱)

MOLP-2/R Cells(提供STR鉴定图谱)

NYSCF-050921-01-MR Cells(提供STR鉴定图谱)

RG-327 Cells(提供STR鉴定图谱)

Ubigene HeLa CCND3 KO Cells(提供STR鉴定图谱)

WTC-mEGFP-DCP1A-cl124 Cells(提供STR鉴定图谱)

HAP1 SS18 (-) 2 Cells(提供STR鉴定图谱)

NIH:OVCAR-5 Cells;背景说明:卵巢癌;腹水转移;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:Ca9-22细胞、HCT-GEO细胞、Hs870T细胞

GM15452 Cells;背景说明:1957年,PuckTT从成年中国仓鼠卵巢的活检组织建立了CHO细胞,CHO-K1是CHO的一个亚克隆。CHO-K1的生长需要脯酸。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:Hs 343.T细胞、Human Hepatocyte Line 5细胞、COLO 320细胞

VeroE6 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:HANK细胞、SF 767细胞、A-20细胞

WM-115 Cells;背景说明:黑色素瘤;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:NCIH1944细胞、MHHCALL2细胞、SK-BR-3细胞

SU-DHL-16 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:RH8994细胞、NPC-TW 01细胞、H184A1细胞

SU-DHL-16 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:RH8994细胞、NPC-TW 01细胞、H184A1细胞

OSC19 Cells;背景说明:舌鳞癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:BL-6细胞、SEG-1细胞、SU86_86细胞

SHSY5Y Cells;背景说明:据报道,该细胞的密度可高达1×106cells/cm2,具有中等水平的多巴胺β羟化酶的活性。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮样;相关产品有:H1666细胞、MEF细胞、FL-83B细胞

18G3.cl 1 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:SNU-216细胞、NCI H157细胞、SKGIIIA细胞

alpha TC1.6 Cells;背景说明:胰岛素瘤;a细胞;C57BL/6xDBA/2;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HO1N1细胞、MIN-6细胞、BC-024细胞

SUDHL2 Cells;背景说明:弥漫性大细胞淋巴瘤;胸腔积液转移;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮;形态特性:详见产品说明书;相关产品有:L-M (TK-)细胞、SV-HUC细胞、NCTC929细胞

SU8686 Cells;背景说明:详见相关文献介绍;传代方法:1:2传代;;生长特性:贴壁生长;形态特性:详见产品说明书;相关产品有:BRL3A细胞、PANC-28细胞、LuCL4细胞

Dysplastic Oral Keratinocyte Cells;背景说明:口腔异常增生;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:ACC2细胞、SK-RC-42细胞、SKMEL1细胞

TFK1 Cells;背景说明:胆管癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:ID8细胞、H520细胞、HCC1143细胞

SU-DH-L5 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:PC 61细胞、RetroPack PT67细胞、EBTr细胞

STSAR-10 Cells(提供STR鉴定图谱)

SDBMSC Cells;背景说明:骨髓间充质干细胞;SD;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:hSCC-25细胞、HSAS4细胞、VMM39细胞

Ca-Ski Cells;背景说明:这株细胞是从小肠肠系膜转移灶的细胞中建立的。 据报道,它含有完整的HPV-16(每个细胞大约600个拷贝)和HPV-18相关序列。;传代方法:1:2传代;生长特性:贴壁生长;形态特性:上皮细胞样;相关产品有:LIM1215细胞、KPNRTBM1细胞、L-1210细胞

COLO-320-DM Cells;背景说明:该细胞可产生5-羟色胺、去甲、、ACTH和甲状旁腺素。角蛋白、波形蛋白弱阳性。培养条件: RPMI 1640  10%FBS;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:悬浮+贴壁;形态特性:淋巴细胞;相关产品有:GEO细胞、KYSE-520细胞、SW1353细胞

MGH-U3 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:RC-K8细胞、AR4-2J细胞、PANC1005细胞

NCTC929 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:OAW-42细胞、GM07404细胞、SK-N-BE(1)n细胞

TALL 1 Cells;背景说明:该细胞源于一名复发T-ALL(急性T淋巴细胞性白血病)的儿童的外周血;具有很强的细胞毒性,体内体外实验中都能破坏肿瘤细胞;IL-2可使细胞更好地生长;α/β TCR阳性,γ/δ TCR阴性;可产生IFNγ、TNF-α和GM-CSF。;传代方法:维持细胞密度在4×105-1×106 cells/ml之间,2-3天换液1次 ;生长特性:悬浮生长;形态特性:淋巴母细胞;相关产品有:Michigan Cancer Foundation-12F细胞、SW837细胞、MPC-5细胞

HCT 116人结肠癌细胞代次低|培养基|送STR图谱

CL11 Cells;背景说明:详见相关文献介绍;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁或悬浮,详见产品说明书部分;形态特性:详见产品说明书;相关产品有:HuH6细胞、P3-X63.Ag8.653细胞、HL7702细胞

SGC996 Cells;背景说明:胆囊癌;女性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HEI-193细胞、HDF-a细胞、SNU-407细胞

H1770 Cells;背景说明:详见相关文献介绍;传代方法:随细胞的生长而换液;生长特性:悬浮生长;形态特性:详见产品说明书;相关产品有:H-209细胞、HuH1细胞、Ku812细胞

GFP-Olig2 Cells;背景说明:胚胎干细胞;129X1/SvJ;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:hTERT-RPE细胞、Tsup-1细胞、H-1435细胞

IM 9 Cells;背景说明:详见相关文献介绍;传代方法:1:3传代,2-3天传一代;生长特性:悬浮生长;形态特性:淋巴母细胞样;相关产品有:3T3 J2细胞、J774细胞、H-4细胞

MDA-MB-435 Cells;背景说明:乳腺癌;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:NCI-H28细胞、LM1细胞、RKO-E6细胞

H-250 Cells;背景说明:小细胞肺癌;男性;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:半贴壁;形态特性:详见产品说明书;相关产品有:DHL10细胞、QGY7701细胞、293-FT细胞

MDAMB435 Cells;背景说明:乳腺癌;传代方法:1:2-1:3传代;每周换液2-3次。;生长特性:贴壁;形态特性:详见产品说明书;相关产品有:HCA-7细胞、THC-8307细胞、MDCC-MSB-1细胞

BayGenomics ES cell line RHA202 Cells(提供STR鉴定图谱)

BayGenomics ES cell line XE022 Cells(提供STR鉴定图谱)

CPTC-TNFRSF9-2 Cells(提供STR鉴定图谱)

MEF_Hsp47 KO-13 Cells(提供STR鉴定图谱)

SK19-4C9 Cells(提供STR鉴定图谱)

LO1.GAK Cells(提供STR鉴定图谱)

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PubMed=26719794; DOI=10.1186/s13742-015-0106-1; PMCID=PMC4696294

Teo A.S.M., Verzotto D., Yao F., Nagarajan N., Hillmer A.M.

Single-molecule optical genome mapping of a human HapMap and a colorectal cancer cell line.

GigaScience 4:65.1-65.6(2015)


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Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A.E.M., Rombouts Y., Wuhrer M.

N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.

Mol. Cell. Proteomics 15:124-140(2016)


PubMed=27377824; DOI=10.1038/sdata.2016.52; PMCID=PMC4932877

Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.

Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.

Sci. Data 3:160052-160052(2016)


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Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Miroo T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)


PubMed=27807467; DOI=10.1186/s13100-016-0078-4; PMCID=PMC5087121

Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.

A map of mobile DNA insertions in the NCI-60 human cancer cell panel.

Mob. DNA 7:20.1-20.11(2016)


PubMed=28179481; DOI=10.1158/1535-7163.MCT-16-0578

Tanaka N., Mashima T., Mizutani A., Sato A., Aoyama A., Gong B., Yoshida H., Muramatsu Y., Nakata K., Matsuura M., Katayama R., Nagayama S., Fujita N., Sugimoto Y., Seimiya H.

APC mutations as a potential biomarker for sensitivity to tankyrase inhibitors in colorectal cancer.

Mol. Cancer Ther. 16:752-762(2017)


PubMed=28192450; DOI=10.1371/journal.pone.0171435; PMCID=PMC5305277

Fasterius E., Raso C., Kennedy S.A., Rauch N., Lundin P., Kolch W., Uhlen M., Al-Khalili Szigyarto C.

A novel RNA sequencing data analysis method for cell line authentication.

PLoS ONE 12:E0171435-E0171435(2017)


PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076

Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)


PubMed=28601559; DOI=10.1016/j.cels.2017.05.009; PMCID=PMC5493283

Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C., Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F., Lindbjerg Andersen C., Nielsen M.L., Olsen J.V.

An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.

Cell Syst. 4:587-599.e4(2017)


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Berg K.C.G., Eide P.W., Eilertsen I.A., Johannessen B., Bruun J., Danielsen S.A., Bjornslett M., Meza-Zepeda L.A., Eknaes M., Lind G.E., Myklebost O., Skotheim R.I., Sveen A., Lothe R.A.

Multi-omics of 34 colorectal cancer cell lines -- a resource for biomedical studies.

Mol. Cancer 16:116.1-116.16(2017)


PubMed=28854368; DOI=10.1016/j.celrep.2017.08.010; PMCID=PMC5583477

Roumeliotis T.I., Williams S.P., Goncalves E., Alsinet C., Del Castillo Velasco-Herrera M., Aben N., Ghavidel F.Z., Michaut M., Schubert M., Price S., Wright J.C., Yu L., Yang M., Dienstmann R., Guinney J.H., Beltrao P., Brazma A., Pardo M., Stegle O., Adams D.J., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Choudhary J.S.

Genomic determinants of protein abundance variation in colorectal cancer cells.

Cell Rep. 20:2201-2214(2017)


PubMed=29101300; DOI=10.15252/msb.20177701; PMCID=PMC5731344

Frejno M., Zenezini Chiozzi R., Wilhelm M., Koch H., Zheng R.-S., Klaeger S., Ruprecht B., Meng C., Kramer K., Jarzab A., Heinzlmeir S., Johnstone E., Domingo E., Kerr D.J., Jesinghaus M., Slotta-Huspenina J., Weichert W., Knapp S., Feller S.M., Kuster B.

Pharmacoproteomic characterisation of human colon and rectal cancer.

Mol. Syst. Biol. 13:951-951(2017)


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Olejniczak A., Szarynska M., Kmiec Z.

In vitro characterization of spheres derived from colorectal cancer cell lines.

Int. J. Oncol. 52:599-612(2018)


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Yuan T.L., Amzallag A., Bagni R., Yi M., Afghani S., Burgan W., Fer N., Strathern L.A., Powell K., Smith B., Waters A.M., Drubin D.A., Thomson T., Liao R., Greninger P., Stein G.T., Murchie E., Cortez E., Egan R.K., Procter L., Bess M., Cheng K.T., Lee C.-S., Lee L.C., Fellmann C., Stephens R., Luo J., Lowe S.W., Benes C.H., McCormick F.

Differential effector engagement by oncogenic KRAS.

Cell Rep. 22:1889-1902(2018)


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Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)


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Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.

Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.

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Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)


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Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. 3rd, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402.e16(2020)


PubMed=32172478; DOI=10.1007/s12253-020-00805-3

Xu Y.-T., Zhang L., Wang Q.-L., Zheng M.-J.

Comparison of different colorectal cancer with liver metastases models using six colorectal cancer cell lines.

Pathol. Oncol. Res. 26:2177-2183(2020)


PubMed=32927768; DOI=10.3390/cancers12092582; PMCID=PMC7564713

Schulte am Esch J., Windmoller B.A., Hanewinkel J., Storm J., Forster C., Wilkens L., Kruger M., Kaltschmidt B., Kaltschmidt C.

Isolation and characterization of two novel colorectal cancer cell lines, containing a subpopulation with potential stem-like properties: treatment options by MYC/NMYC inhibition.

Cancers (Basel) 12:2582.1-2582.34(2020)"


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