ARF3抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-6: Mouse brain tissue, Human fetal brain tissue, 231, Raji, HepG2 and Hela cell lysates, Primary antibody: P06309(ARF3 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P06309(ARF3 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P06309(ARF3 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The ADP-ribosylation factor 3 (ARF3) antibody is a research tool designed to detect and study ARF3. a small GTPase belonging to the ARF family. ARF proteins are critical regulators of intracellular vesicular trafficking, membrane remodeling, and cytoskeletal organization. ARF3. classified as a Class II ARF protein, is primarily localized to the Golgi apparatus and plasma membrane, where it facilitates cargo transport, lipid metabolism, and organelle structure maintenance. Unlike Class I ARFs (e.g., ARF1), ARF3 lacks the N-terminal myristoylation site, influencing its membrane association dynamics.

ARF3-specific antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, subcellular localization, and interactions with effector proteins such as coatomer complexes and lipid-modifying enzymes. These studies help unravel ARF3's role in cellular processes like secretion, endocytosis, and cell signaling. Dysregulation of ARF3 has been implicated in pathological conditions, including cancer and neurodegenerative disorders, making its antibody a valuable tool for mechanistic and diagnostic research. Commercial ARF3 antibodies are typically raised in rabbits or mice, validated for specificity against recombinant or endogenous proteins across human, mouse, and rat models.