ZNF76抗体—艾普蒂

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: K562 cell lysate, Primary antibody: P05964(ZNF76 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P05964(ZNF76 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The ZNF76 antibody is a valuable tool for studying the Zinc Finger Protein 76 (ZNF76), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. ZNF76. also known as HUB1. is a nuclear protein encoded in humans by the ZNF76 gene on chromosome 16. It contains an N-terminal KRAB domain, which mediates transcriptional repression, and C-terminal C2H2-type zinc finger motifs for DNA binding. ZNF76 functions as a transcriptional repressor, interacting with heterochromatin protein 1 (HP1) to regulate chromatin organization and gene silencing. It plays roles in cell cycle regulation, differentiation, and development.

The antibody is widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect ZNF76's expression, localization, and DNA-binding activity. It recognizes endogenous ZNF76 across species, including human, mouse, and rat. Research utilizing this antibody has linked ZNF76 to transcriptional regulation of genes involved in stress response and tumor suppression. Notably, studies suggest its dysregulation may contribute to carcinogenesis, with reduced expression observed in certain cancers. Commercial ZNF76 antibodies are typically developed using immunogens corresponding to specific epitopes within the protein's conserved regions, ensuring specificity for targeted applications in molecular and cellular biology research.