IQGAP1抗体—艾普蒂

+ +

•  

   

     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: HUVEC cell lysate, Primary antibody: P05889(IQGAP1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P05889(IQGAP1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

IQGAP1 (IQ motif-containing GTPase-activating protein 1) is a ubiquitously expressed scaffolding protein involved in regulating diverse cellular processes, including cytoskeletal organization, cell-cell adhesion, and signal transduction. It interacts with multiple partners, such as Rho-family GTPases (e.g., Cdc42. Rac1), β-catenin, E-cadherin, and components of the MAPK pathway, playing critical roles in cell migration, proliferation, and polarity. Dysregulation of IQGAP1 is implicated in cancer progression, neurodevelopmental disorders, and inflammatory diseases, making it a focal point in biomedical research.

Antibodies targeting IQGAP1 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to investigate its role in health and disease. Commercial IQGAP1 antibodies are typically raised against specific epitopes within its functional domains (e.g., CHD, GRD, or RasGAP domains) and vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and reactivity (human, mouse, rat). Validation includes testing for specificity via knockout cell lines or siRNA-mediated depletion. Researchers must select antibodies based on intended applications, as cross-reactivity with IQGAP2/3 or other proteins may occur. Reliable IQGAP1 antibodies aid in unraveling its complex regulatory networks and therapeutic potential.