HOXB5抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HUVEC and Jarkat cell lysates, Primary antibody: P05866(HOXB5 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

The HOXB5 antibody is a tool used to detect the Homeobox B5 (HOXB5) protein, a member of the homeobox (HOX) gene family critical in embryonic development and cellular differentiation. HOX genes encode transcription factors that regulate body patterning and organogenesis by controlling gene expression. Specifically, HOXB5. located on chromosome 17. plays roles in neural, cardiovascular, and respiratory system development. Dysregulation of HOXB5 has been implicated in cancers (e.g., breast, colorectal, lung) due to its influence on cell proliferation, apoptosis, and metastasis.

HOXB5 antibodies are widely used in research to study protein localization, expression levels, and functional mechanisms via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes of the HOXB5 protein. Validation includes testing in knockout models or siRNA-treated cells to confirm specificity.

Recent studies highlight HOXB5's potential as a diagnostic or prognostic biomarker in oncology and regenerative medicine. For example, elevated HOXB5 correlates with poor survival in certain cancers, while its role in stem cell differentiation underscores therapeutic applications. Researchers must select antibodies validated for their specific experimental conditions, as cross-reactivity with other HOX proteins can occur. Commercial suppliers often provide detailed protocols and validation data to ensure reliability.