GAP43抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human cerebrum tissue, Mouse heart tissue lysates, Primary antibody: P05855(GAP43 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P05855(GAP43 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P05855(GAP43 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

**Background of GAP43 Antibody**

The Growth Associated Protein 43 (GAP43), also known as neuromodulin or B-50. is a neuron-specific protein crucial for axonal growth, synaptic plasticity, and nerve regeneration. It is highly expressed during neuronal development, particularly in growing axons and growth cones, and its levels decline in most mature neurons. However, GAP43 re-emerges during nerve injury or regeneration, making it a key marker for studying neurodevelopmental processes and repair mechanisms.

GAP43 antibodies are widely used to detect this protein in research applications, including immunohistochemistry (IHC), Western blotting, and immunofluorescence (IF). These antibodies help investigate neural development, synaptic remodeling, and responses to neural injury. GAP43’s role in modulating intracellular signaling pathways, such as protein kinase C (PKC) activity, further links it to learning, memory, and neurodegenerative disorders like Alzheimer’s and Parkinson’s diseases.

Structurally, GAP43 is a 24 kDa protein with a conserved palmitoylation site for membrane anchoring and a PKC phosphorylation domain. Antibodies targeting specific epitopes (e.g., phosphorylated residues) enable functional studies of its post-translational modifications. Both monoclonal and polyclonal GAP43 antibodies are available, with validation often including knockout controls to ensure specificity.

Overall, GAP43 antibodies serve as essential tools in neuroscience, offering insights into neural repair, developmental biology, and disease mechanisms.