POLH抗体—艾普蒂

+ +

•  

   

     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-5: A172, 231, Jurkat, HepG2 and Hela cell lysates, Primary antibody: P02902(POLH Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P02902(POLH Antibody) at dilution 1/45. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P02902(POLH Antibody) at dilution 1/45. (Original magnification: ×200)    

  
  

POLH antibody targets the POLH protein, also known as DNA polymerase eta (Pol η), a specialized enzyme involved in DNA damage repair. Belonging to the Y-family of translesion synthesis (TLS) polymerases, POLH facilitates error-prone replication across ultraviolet (UV)-induced DNA lesions, such as cyclobutane pyrimidine dimers (CPDs), which distort the DNA helix. This bypass mechanism, termed error-prone repair, allows cells to tolerate damage and continue replication, albeit with a higher mutation risk. POLH mutations are linked to xeroderma pigmentosum variant (XP-V), a condition characterized by extreme UV sensitivity and heightened skin cancer susceptibility due to defective TLS.

POLH antibodies are essential tools in studying DNA repair mechanisms, protein interactions, and POLH expression patterns in normal versus cancerous tissues. They enable detection via techniques like Western blot, immunohistochemistry (IHC), and immunofluorescence (IF), aiding research on POLH's role in replication stress responses, chemotherapy resistance, and mutagenesis. These antibodies also help assess POLH dysfunction in XP-V diagnostics and explore therapeutic strategies targeting TLS pathways in oncology.