POLR2I抗体—艾普蒂

The POLR2I antibody targets the POLR2I protein, a subunit of RNA polymerase II (Pol II), which is essential for eukaryotic transcription. POLR2I, also known as RPB9. plays a critical role in transcription initiation and elongation by contributing to the structural integrity of Pol II and participating in promoter recognition. This subunit is implicated in transcription-coupled DNA repair and may influence transcriptional fidelity by aiding in proofreading during RNA synthesis. Antibodies against POLR2I are widely used in research to study Pol II assembly, transcriptional regulation, and its dysregulation in diseases like cancer or neurodegenerative disorders. They enable detection of POLR2I expression and localization via techniques such as Western blotting, immunofluorescence, and ChIP-seq. POLR2I antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Commercial antibodies are typically raised in rabbits or mice, with applications spanning basic molecular biology and clinical studies exploring transcriptional anomalies. Understanding POLR2I's function through these antibodies provides insights into gene expression mechanisms and potential therapeutic targets.