HOOK2抗体—艾普蒂

+ +

•  

   

     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

•  

   

     Western blot analysis using FTL mouse mAb against HepG2 (1), K562 (2) cell lysate.    

  
  

•  

   

     Flow cytometric analysis of HepG2 cells using FTL mouse mAb (green) and negative control (red).    

  
  

•  

   

     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using FTL mouse mAb with DAB staining.    

  
  

•  

   

     Immunohistochemical analysis of paraffin-embedded human esophageal tissues using FTL mouse mAb with DAB staining.    

  
  

The HOOK2 antibody is a valuable tool in studying the HOOK family of proteins, which play critical roles in intracellular trafficking processes. HOOK2. a member of the HOOK protein family (HOOK1. HOOK2. HOOK3), acts as an adaptor protein that links organelles to microtubules, facilitating vesicular transport and organelle positioning. It is structurally characterized by an N-terminal microtubule-binding domain, a central coiled-coil region, and a C-terminal domain that interacts with cargo-specific proteins. HOOK2 is particularly implicated in endosomal trafficking, Golgi organization, and ciliogenesis, where it collaborates with molecular partners like FTS (Fused Toes) and RUN domain proteins to regulate cargo movement along microtubules.

Research using HOOK2 antibodies has highlighted its functional significance in diverse cellular processes, including receptor recycling, lysosomal biogenesis, and centrosome cohesion. Dysregulation of HOOK2 expression or mutations are associated with pathological conditions such as ciliopathies, neurodevelopmental disorders, and cancers. For instance, reduced HOOK2 levels correlate with defective cilia formation, contributing to renal or retinal diseases. In cancer, altered HOOK2 expression may influence cell migration and metastasis via disrupted intracellular transport.

HOOK2-specific antibodies enable the detection and localization of endogenous HOOK2 in cells and tissues through techniques like Western blot, immunofluorescence, and immunohistochemistry. These reagents are essential for exploring HOOK2's molecular interactions, post-translational modifications, and tissue-specific expression patterns, providing insights into its physiological and pathological roles across species, including humans and model organisms.