QTRT1抗体—艾普蒂

+ +

•  

   

     Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    

  
  

•  

   

     Immunofluorescence analysis of NIH/3T3 cells using FGG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    

  
  

The QTRT1 (queuine tRNA-ribosyltransferase catalytic subunit 1) antibody is a valuable tool in molecular biology research for investigating the function and expression of the QTRT1 protein. QTRT1. also known as tRNA-guanine transglycosylase (TGT), is an enzyme critical for post-transcriptional modification of transfer RNA (tRNA). It catalyzes the exchange of guanine with queuine, a hypermodified nucleobase, at the wobble position of specific tRNAs (e.g., tRNA-Asp, -Asn, -His, -Tyr), enhancing translational accuracy and efficiency. This modification is conserved across eukaryotes and plays roles in cellular stress responses, development, and disease pathogenesis.

QTRT1 antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression levels, subcellular localization, and tissue distribution. Studies have linked QTRT1 dysregulation to neurological disorders, cancer, and developmental anomalies, underscoring its biological significance. For instance, reduced QTRT1 expression has been observed in certain brain tumors and neurodegenerative conditions, while mutations are associated with intellectual disability and microcephaly in model organisms.

Researchers utilize QTRT1 antibodies to explore its interaction partners, regulatory mechanisms, and potential therapeutic targets. Commercial antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. As queuine modification is diet-dependent in mammals, QTRT1 studies also intersect with nutrition and metabolic research. Overall, QTRT1 antibodies serve as essential reagents for dissecting the molecular pathways governed by tRNA modification and their implications in health and disease.