DDX27抗体—艾普蒂

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: HEPG2, Hela and K562 cell lysates, Primary antibody: P02610(DDX27 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P02610(DDX27 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The DDX27 antibody is a research tool designed to detect and study the DEAD-box helicase 27 (DDX27) protein, a member of the DEAD-box RNA helicase family. DDX27 is involved in various RNA-related processes, including ribosome biogenesis, RNA splicing, and translation regulation. It plays a critical role in cellular homeostasis by binding to RNA and unwinding secondary structures during RNA maturation and degradation. Dysregulation of DDX27 has been implicated in diseases such as cancer and neurodegenerative disorders, making it a target for mechanistic and therapeutic investigations.

The DDX27 antibody is typically developed in host species like rabbits or mice using immunogenic peptides or recombinant protein fragments. It enables researchers to analyze DDX27 expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Validated antibodies are essential for ensuring specificity, minimizing cross-reactivity with other DEAD-box proteins. Recent studies have utilized DDX27 antibodies to explore its role in ribosomal RNA processing and its potential as a biomarker in cancers, including colorectal and hepatocellular carcinomas.

As a reagent, the DDX27 antibody supports fundamental research into RNA metabolism mechanisms and disease pathways, aiding the development of targeted therapies. Commercial availability and validation data (e.g., species reactivity, application suitability) are critical considerations for experimental reproducibility.