RCOR3抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: 293T, Jurkat and Hela cell, Mouse brain tissue, NIH/3T3 cell lysates, Primary antibody: P02553(RCOR3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P02553(RCOR3 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The RCOR3 antibody is a tool used to detect the REST Corepressor 3 (RCOR3) protein, a member of the RCOR family (RCOR1. RCOR2. RCOR3) that interacts with the repressor element-1 silencing transcription factor (REST). RCOR3 functions as a transcriptional co-repressor, primarily by forming complexes with REST and chromatin-modifying enzymes, such as histone demethylases (e.g., LSD1/KDM1A) and histone deacetylases (HDACs). This interaction facilitates chromatin remodeling, leading to gene silencing, particularly in neural and developmental contexts. RCOR3 is implicated in regulating neuronal gene expression, stem cell differentiation, and cellular responses to stress.

Unlike RCOR1 and RCOR2. RCOR3 exhibits tissue-specific expression, with higher levels observed in the brain, testis, and certain cancers. Its role in cancer remains debated, with studies suggesting both tumor-suppressive and oncogenic functions depending on cellular context. For example, RCOR3 may promote neuroblastoma progression by modulating REST-dependent gene networks, while its loss has been linked to genomic instability in other cancers.

RCOR3 antibodies are widely used in research to study protein localization (via immunohistochemistry or immunofluorescence), expression levels (via Western blot), and protein-DNA interactions (via ChIP-seq). Validation of these antibodies often involves knockout cell lines or tissues to confirm specificity. Commercially available RCOR3 antibodies vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and applications, requiring careful selection based on experimental needs.