UBE2M抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela, Raji, LO2 cell lysates, Primary antibody: P02344(UBE2M Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P02344(UBE2M Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

UBE2M (Ubiquitin-Conjugating Enzyme E2 M), also known as UBC12. is a member of the E2 ubiquitin-conjugating enzyme family that plays a critical role in the neddylation pathway. This post-translational modification involves the covalent attachment of NEDD8 (neural precursor cell-expressed developmentally downregulated protein 8) to target proteins, primarily the Cullin subunits of Cullin-RING E3 ubiquitin ligases (CRLs). Neddylation activates CRLs, enabling their function in ubiquitinating substrate proteins for proteasomal degradation, thereby regulating processes like cell cycle progression, DNA repair, and stress responses.

UBE2M antibodies are essential tools for studying neddylation dynamics, protein turnover, and CRL activity. These antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to detect UBE2M expression levels, localization, and interactions with partners like NAE1 (NEDD8-activating enzyme) or Cullins. Researchers also employ UBE2M antibodies to investigate dysregulation of the neddylation pathway in diseases, including cancers, neurodegenerative disorders, and immune dysfunctions, where CRL activity is often altered. Both monoclonal and polyclonal variants are available, typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. Host species (e.g., rabbit, mouse) and clonality are selected based on experimental requirements, ensuring reliable reproducibility in diverse biological models.