TRAFD1抗体—艾普蒂

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using TRAFD1 mouse mAb against HEK293 (1), Raji (2), and Jurkat (3) cell lysate.    

  
  

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     Immunofluorescence analysis of HepG2 cells using TRAFD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.    

  
  

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     Flow cytometric analysis of HeLa cells using TRAFD1 mouse mAb (green) and negative control (purple).    

  
  

**Background of TRAFD1 Antibody**

TRAFD1 (TRAF-type zinc finger domain-containing protein 1) is a member of the TRAF (TNF receptor-associated factor) protein family, which plays critical roles in immune signaling pathways. Unlike most TRAF proteins involved in pro-inflammatory signaling, TRAFD1 acts as a negative regulator of innate immune responses, particularly in modulating Toll-like receptor (TLR) and RIG-I-like receptor (RLR) pathways. It suppresses excessive interferon (IFN) production by promoting the degradation of key signaling molecules, such as MAVS, thereby maintaining immune homeostasis.

TRAFD1 antibodies are essential tools for studying its expression, localization, and functional interactions in immune regulation. These antibodies enable detection of TRAFD1 in techniques like Western blotting, immunoprecipitation, and immunofluorescence, aiding research into its role in viral infections, autoimmune diseases, and cancer. Commercial TRAFD1 antibodies are typically raised in rabbits or mice, with validation in knockout cell lines to ensure specificity. Dysregulation of TRAFD1 has been linked to pathologies like chronic inflammation and tumorigenesis, highlighting its potential as a therapeutic target. Research using TRAFD1 antibodies continues to uncover its complex regulatory mechanisms, bridging gaps in understanding immune balance and disease progression.