GNL3抗体—艾普蒂

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate.    

  
  

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     Flow cytometric analysis of Jurkat cells using GNL3 mouse mAb (green) and negative control (purple).    

  
  

**Background of GNL3 Antibody**

GNL3 (Guanine Nucleotide-Binding Protein-Like 3), also known as nucleostemin (NS), is a nucleolar GTP-binding protein implicated in cell proliferation, stem cell maintenance, and ribosome biogenesis. It is highly expressed in stem cells, embryonic tissues, and cancer cells, but downregulated in differentiated cells, suggesting a role in sustaining undifferentiated states. GNL3 regulates cell cycle progression by interacting with p53 pathways; it binds to MDM2. inhibiting p53 stabilization and delaying apoptosis under stress conditions. This mechanism supports cell survival and proliferation, particularly in rapidly dividing cells.

GNL3 antibodies are essential tools for studying its expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding research on its role in cancer biology, stem cell dynamics, and regenerative medicine. Elevated GNL3 levels correlate with poor prognosis in cancers (e.g., gliomas, hepatocellular carcinoma), making it a potential diagnostic or therapeutic target. Antibodies against GNL3 also help explore its interactions with nucleolar components and GTP-dependent regulatory mechanisms. Ongoing studies focus on targeting GNL3 to disrupt cancer cell proliferation or enhance tissue regeneration, highlighting its dual significance in disease and development.