PCNA抗体—艾普蒂

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     Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);    

  
  

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     Western blot analysis using PCNA mouse mAb against A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate.    

  
  

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     Western blot analysis using PCNA mouse mAb against 81505(1),KO-SF(2),RSC-96(3),NIH/3T3(4) cell lysate.    

  
  

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     Flow cytometric analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using PCNA mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using PCNA mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Mouse testis(A)Mouse stomach(B)Mouse pancreas(C) using PCNA mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rat testis(A)Rat thyroid(B)Rat colon(C) using PCNA mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded Rabbit liver(A)Rabbit colon(B) using PCNA mouse mAb with DAB staining.    

  
  

Proliferating Cell Nuclear Antigen (PCNA) is a highly conserved, ring-shaped protein that plays a central role in DNA replication, repair, and cell cycle regulation. Acting as a sliding clamp, PCNA forms a homotrimeric complex that encircles DNA, providing a platform for recruiting enzymes such as DNA polymerases, ligases, and helicases. Its expression peaks during the S phase of the cell cycle, making it a widely used marker for proliferating cells.

PCNA-specific antibodies are essential tools in biomedical research and diagnostics. They enable the detection and quantification of proliferating cells in tissues, particularly in cancer studies, where PCNA levels correlate with tumor aggressiveness and prognosis. These antibodies are employed in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to assess cell proliferation status. Monoclonal antibodies (e.g., clone PC10) are commonly used due to their high specificity.

Beyond basic research, PCNA antibodies aid in understanding DNA damage response mechanisms, as PCNA undergoes post-translational modifications (e.g., ubiquitination) to recruit repair proteins. Challenges include cross-reactivity with bacterial proteins in some applications and variability in staining protocols. Despite this, PCNA remains a cornerstone biomarker for studying cell proliferation dynamics in health and disease.