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  • ATM抗体—艾普蒂
  • ATM抗体—艾普蒂
  • ATM抗体—艾普蒂

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ATM抗体—艾普蒂

Mouse Monoclonal ATM Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-16

上海切尔齐生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：ruihening88@163.com

产品详情:

中文名称：
ATM抗体
英文名称：
Mouse Monoclonal ATM Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 1552 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
ATM

验证与应用

应用及物种
WB咨询技术 Human, Mouse, Monkey, Rat
IF咨询技术 Human, Mouse, Monkey, Rat
IHC1/200 - 1/1000 Human, Mouse, Monkey, Rat
ICC1/200 - 1/1000 Human, Mouse, Monkey, Rat
FCM1/200 - 1/400 Human, Mouse, Monkey, Rat
Elisa1/10000 Human, Mouse, Monkey, Rat
   

产品详情

AliasesAT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
Entrez GeneID472
clone3H12H8
WB Predicted band size350.7kDa
Host/IsotypeMouse IgG2b
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Monkey, Rat
ImmunogenPurified recombinant fragment of human ATM (AA: 2577-3056) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of Hela cells using ATM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunofluorescence analysis of NIH/3T3 cells using ATM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using ATM mouse mAb (green) and negative control (red).    


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       Flow cytometric analysis of COS-7 cells using ATM mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human kidney tissues using ATM mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human gastric cancer tissues using ATM mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Mouse spleen tissues using ATM mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rat myocardium tissues using ATM mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded Rabbit spleen tissues using ATM mouse mAb with DAB staining.    


           

参考文献

以下是关于ATM抗体的3篇参考文献，涵盖其应用机制及疾病研究：

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1. **标题**: *ATM-dependent phosphorylation of the checkpoint kinase CHK2 in response to DNA damage*

**作者**: Ahn, J.Y., Schwarz, J.K., Piwnica-Worms, H., & Canman, C.E.

**摘要**: 该研究利用ATM抗体验证ATM蛋白在DNA损伤后对CHK2激酶的磷酸化调控作用，揭示了ATM通过激活下游信号通路参与细胞周期检查点调控的机制。

2. **标题**: *Defective kinase activity and DNA repair in ATM-deficient cells*

**作者**: Lavin, M.F., & Shiloh, Y.

**摘要**: 通过ATM抗体检测ATM缺失细胞系中的蛋白表达，发现ATM激酶活性缺陷导致DNA双链断裂修复异常，解释了共济失调毛细血管扩张症(AT)患者的基因组不稳定性。

3. **标题**: *Expression and functional analysis of ATM variants in cancer progression*

**作者**: Sun, Y., Jiang, X., & Chen, J.

**摘要**: 研究采用ATM抗体分析多种癌症组织中的ATM蛋白表达水平，发现ATM表达缺失与肿瘤对放化疗敏感性相关，为靶向ATM的癌症治疗提供依据。

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以上文献可通过PubMed或Google Scholar检索原文。如需更近期研究，建议结合关键词“ATM antibody”及“2020-2023”筛选。

       

背景信息

ATM antibodies target the Ataxia-Telangiectasia Mutated (ATM) protein, a critical serine/threonine kinase involved in DNA damage response (DDR). Discovered in studies of the autosomal recessive disorder Ataxia-Telangiectasia (A-T), ATM is activated by double-strand DNA breaks (DSBs) and coordinates cell cycle checkpoints, DNA repair, and apoptosis. Mutations in the ATM gene cause A-T, characterized by neurodegeneration, immune dysfunction, and heightened cancer risk.

ATM antibodies are widely used in research to detect ATM expression, phosphorylation status (e.g., active ATM phosphorylates substrates like CHK2 and p53), and subcellular localization via techniques like Western blotting or immunofluorescence. They also aid in diagnosing A-T by identifying reduced or dysfunctional ATM protein in patient cells. Beyond A-T, ATM dysregulation is implicated in cancers (e.g., leukemia, breast cancer) and radiation sensitivity studies, as ATM-deficient cells fail to repair radiation-induced DSBs. Recent therapeutic efforts focus on ATM inhibitors to sensitize cancer cells to radiotherapy or chemotherapy. However, ATM's dual role in tumor suppression and treatment resistance complicates its targeting. Research continues to unravel ATM's interactions with DDR pathways, its non-canonical roles in oxidative stress, and potential as a biomarker or therapeutic target.

       
ATM抗体;ATM;ATM Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业，

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,蛋白组学,细胞生物学

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