PDGFA抗体—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using PDGFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Immunofluorescence analysis of NIH/3T3 cells using PDGFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Jurkat cells using PDGFA mouse mAb (green) and negative control (red).    

  
  

The platelet-derived growth factor alpha (PDGFA) antibody is a crucial tool in biomedical research for detecting and analyzing the expression, localization, and function of the PDGFA protein. PDGFA is a member of the platelet-derived growth factor (PDGF) family, which plays essential roles in cell growth, proliferation, differentiation, and angiogenesis. Specifically, PDGFA binds to its receptor PDGFR-α, activating downstream signaling pathways such as MAPK and PI3K/Akt, which regulate processes like tissue repair, embryonic development, and vascular formation. Dysregulation of PDGFA signaling is implicated in various pathologies, including cancer progression, atherosclerosis, and organ fibrosis, making it a key target for therapeutic and diagnostic studies.

PDGFA antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein levels in tissues or cell lines. Researchers employ these antibodies to investigate PDGFA's role in disease mechanisms, evaluate its overexpression in tumors, or monitor therapeutic responses in preclinical models. Additionally, PDGFA antibodies aid in studying interactions with other signaling molecules and validating genetic or pharmacological interventions targeting the PDGF pathway. Given the heterogeneity of PDGFA isoforms and post-translational modifications, selecting antibodies with high specificity and validated performance is critical. Many commercial PDGFA antibodies are raised against specific epitopes, with host species (e.g., rabbit, mouse) and clonal variations (monoclonal/polyclonal) offering flexibility for diverse experimental needs. Proper validation via knockout controls or siRNA knockdown ensures reliable results, underscoring their importance in both basic and translational research.