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  • IRF3抗体—艾普蒂
  • IRF3抗体—艾普蒂
  • IRF3抗体—艾普蒂

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IRF3抗体—艾普蒂

Mouse Monoclonal IRF3 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-16

上海切尔齐生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：ruihening88@163.com

产品详情:

中文名称：
IRF3抗体
英文名称：
Mouse Monoclonal IRF3 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 1542 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
IRF3

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesIIAE7
Entrez GeneID3661
clone2A10A8
WB Predicted band size47.2kDa
Host/IsotypeMouse IgG2b
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenPurified recombinant fragment of human IRF3 (AA: 1-150) expressed in HEK293-6e cells supernatant.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Immunofluorescence analysis of Hela cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunofluorescence analysis of NIH/3T3 cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunofluorescence analysis of RSC96 cells using IRF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using IRF3 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using IRF3 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using IRF3 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using IRF3 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using IRF3 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded mouse liver tissues using IRF3 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using IRF3 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded rat liver tissues using IRF3 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded rat kidney tissues using IRF3 mouse mAb with DAB staining.    


           

参考文献

以下是关于IRF3抗体的3篇参考文献及其摘要概括：

1. **文献名称**：*"IRF3 activation via phosphorylation by the antiviral kinase TBK1"*

**作者**：Sato, M. et al.

**摘要**：研究阐明了IRF3在抗病毒免疫中的激活机制，通过TBK1介导的磷酸化促进其核转位。实验中利用IRF3抗体检测其磷酸化状态及亚细胞定位。

2. **文献名称**：*"Viral recognition by TLRs and cytoplasmic RNA sensors: role of IRF3 in type I IFN responses"*

**作者**：Honda, K. et al.

**摘要**：探讨病毒识别通路中IRF3的核心作用，通过Western blot和免疫荧光技术(使用IRF3抗体)揭示其在TLR和RLR信号中的激活动态。

3. **文献名称**：*"Regulation of IRF3 activation in antiviral innate immunity"*

**作者**：Servant, M.J. et al.

**摘要**：分析IRF3的翻译后修饰(如泛素化)对抗病毒反应的影响，通过IRF3抗体进行免疫共沉淀实验，揭示其与下游信号分子的相互作用。

4. **文献名称**：*"IRF3-dependent cross-talk between innate antiviral and DNA damage responses"*

**作者**：Zeng, W. et al.

**摘要**：研究IRF3在DNA损伤与抗病毒信号中的双重角色，使用IRF3抗体验证其在DNA损伤条件下的异常激活及功能关联。

这些文献均通过IRF3抗体探究其在先天免疫中的分子机制，涵盖磷酸化检测、亚细胞定位及蛋白互作分析等应用场景。

       

背景信息

Interferon Regulatory Factor 3 (IRF3) is a key transcription factor in innate immune responses, particularly in antiviral signaling. It is normally sequestered in the cytoplasm in an inactive form. Upon viral infection or detection of pathogen-associated molecular patterns (PAMPs), pattern recognition receptors (e.g., TLR3. RIG-I) trigger signaling cascades that lead to IRF3 phosphorylation. This post-translational modification enables IRF3 dimerization, nuclear translocation, and activation of type I interferons (IFNs) and pro-inflammatory cytokines, critical for host defense.

IRF3 antibodies are essential tools for studying its expression, activation status, and subcellular localization. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate IRF3's role in immune signaling pathways. Phospho-specific IRF3 antibodies (e.g., recognizing Ser386 phosphorylation) are particularly valuable for distinguishing activated IRF3 from its inactive form. Researchers also employ these antibodies to explore IRF3 dysregulation in diseases, including viral evasion mechanisms, autoimmune disorders, and cancer immunotherapy studies. However, users must validate antibody specificity due to potential cross-reactivity with homologous proteins (e.g., IRF7) and ensure proper controls (e.g., IRF3-knockout cells) in experiments.

       
IRF3抗体;IRF3;IRF3 Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业，

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,蛋白组学,细胞生物学

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