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NPM3抗体—艾普蒂新品

Mouse Monoclonal NPM3 Antibody

询价 20μl 起订

50μl 起订

100μl 起订

上海更新日期：2025-05-16

上海切尔齐生物科技有限公司

非会员

联系人：周***

电话：027***

手机：180***

邮箱：rui***

产品详情:

中文名称：: NPM3抗体

英文名称：: Mouse Monoclonal NPM3 Antibody

品牌：: 艾普蒂

产地：: 武汉

保存条件：: Stored at -20°C for 1537 year. Avoid repeated freeze / thaw cycles.

产品类别：: 抗体

重组：: 否

应用：: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA

种属反应性：: Human,Mouse,Rat

宿主：: Mouse

偶联物：: 无

靶点：: NPM3

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/200 - 1/1000	Human,Mouse,Rat
ICC	1/50 - 1/200	Human,Mouse,Rat
FCM	1/200 - 1/400	Human,Mouse,Rat
Elisa	1/10000	Human,Mouse,Rat

产品详情

Aliases	PORMIN; TMEM123
Entrez GeneID	10360
clone	2A3B1
WB Predicted band size	19kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Purified recombinant fragment of human NPM3 (AA: full 1-178) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

+ +

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis of Hela cells using NPM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hek293 cells using NPM3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using NPM3 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using NPM3 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded mouse brain tissues using NPM3 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using NPM3 mouse mAb with DAB staining.

参考文献

以下是关于NPM3抗体的3篇参考文献示例(注：以下为模拟内容，具体文献需根据实际检索补充)：

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1. **文献名称**：*NPM3 regulates ribosome biogenesis and cell proliferation via rRNA processing*

**作者**：Smith A, et al.

**摘要**：研究揭示NPM3在核糖体RNA加工中的关键作用，通过敲除实验和NPM3特异性抗体(Western blot/免疫荧光)验证其与核仁蛋白的相互作用，表明其异常表达与细胞周期失调相关。

2. **文献名称**：*Development of a monoclonal antibody against NPM3 for cancer diagnostics*

**作者**：Chen L, et al.

**摘要**：报道一种新型NPM3单克隆抗体的开发与验证，用于免疫组化检测多种肿瘤组织中NPM3的过表达，提示其作为癌症生物标志物的潜力。

3. **文献名称**：*NPM3 deficiency disrupts embryonic stem cell differentiation*

**作者**：Wang Y, et al.

**摘要**：利用NPM3抗体进行染色质免疫沉淀(ChIP)和蛋白定位分析，发现NPM3缺失导致胚胎干细胞多能性相关基因表达异常，影响分化过程。

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如需具体文献，建议通过PubMed或Google Scholar检索关键词“NPM3 antibody”或“Nucleophosmin 3 antibody”获取最新研究。

背景信息

NPM3 (Nucleophosmin/Nucleoplasmin 3) is a member of the nucleophosmin/nucleoplasmin family, which plays critical roles in chromatin remodeling, histone chaperoning, and ribosome biogenesis. Unlike its well-studied homolog NPM1 (linked to cancer and ribosome assembly), NPM3 remains less characterized but shares structural features, including acidic domains for histone binding and nucleolar localization signals. It is implicated in early embryonic development, oocyte maturation, and epigenetic regulation, potentially through interactions with histones or transcription factors.

NPM3 antibodies are essential tools for studying its expression, localization, and molecular interactions. They enable detection via techniques like Western blot, immunofluorescence, and immunohistochemistry, aiding research on its role in cellular processes and diseases. Dysregulation of NPM3 has been tentatively associated with cancer (e.g., gliomas, leukemia) and developmental disorders, though mechanistic insights remain limited. Commercial antibodies typically target specific epitopes, such as the N-terminal region, and require validation for species cross-reactivity (human, mouse, rat). Recent studies also explore NPM3’s potential as a biomarker or therapeutic target, emphasizing the need for reliable antibodies to decipher its biological and pathological significance.

NPM3抗体;NPM3;NPM3 Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业，

成立日期	(2年)
注册资本	20万人民币
员工人数	1-10人
年营业额	￥ 100万以内
经营模式	贸易,工厂,试剂,定制,服务
主营行业	抗体,蛋白组学,细胞生物学