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  • MCM3抗体—艾普蒂
  • MCM3抗体—艾普蒂
  • MCM3抗体—艾普蒂

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MCM3抗体—艾普蒂

Mouse Monoclonal MCM3 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-16

上海切尔齐生物科技有限公司

非会员
联系人：周***
电话：027***
手机：180***
邮箱：rui***

产品详情:

中文名称：
MCM3抗体
英文名称：
Mouse Monoclonal MCM3 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 1528 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
MCM3

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Rat,Monkey
IF咨询技术 Human,Rat,Monkey
IHC1/200 - 1/1000 Human,Rat,Monkey
ICC1/50 - 1/200 Human,Rat,Monkey
FCM1/200 - 1/400 Human,Rat,Monkey
Elisa咨询技术 Human,Rat,Monkey
   

产品详情

AliasesHCC5; P1.h; RLFB; P1-MCM3
Entrez GeneID4172
clone1D4A3
WB Predicted band size91kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Rat,Monkey
ImmunogenPurified recombinant fragment of human MCM3 expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

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       Western blot analysis using MCM3 mouse mAb against PC-12 (1), HepG2 (2),Hela (3) and COS7 (4) cell lysate.    


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       Immunofluorescence analysis of Hela cells using MCM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


  •  

     

       Flow cytometric analysis of Hela cells using MCM3 mouse mAb (green) and negative control (red).    


  •  

     

       Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using MCM3 mouse mAb with DAB staining.    


  •  

     

       Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using MCM3 mouse mAb with DAB staining.    


           

参考文献

以下是关于MCM3抗体的3篇参考文献及其摘要概括：

1. **文献名称**：*"Immunohistochemical analysis of minichromosome maintenance protein 3 (MCM3) in human colorectal cancer tissues"*

**作者**：Giaginis et al.

**摘要**：该研究通过免疫组化技术分析了MCM3在结直肠癌组织中的表达水平，发现MCM3在癌细胞中显著高表达，并与肿瘤分期和患者不良预后相关，提示其可作为潜在的癌症生物标志物。

2. **文献名称**：*"MCM3 as a novel proliferation marker in human gliomas: Comparison with Ki-67"*

**作者**：Wharton et al.

**摘要**：本文比较了MCM3抗体与Ki-67在神经胶质瘤中的检测效果，发现MCM3在增殖细胞中的表达更敏感且稳定，建议其作为评估脑肿瘤细胞增殖活性的可靠指标。

3. **文献名称**：*"Cell cycle-regulated phosphorylation of MCM3: Role in chromatin binding and ATPase activity"*

**作者**：Ishimi et al.

**摘要**：研究探讨了MCM3在细胞周期中的磷酸化修饰及其功能，通过抗体检测发现其磷酸化状态影响与染色质的结合及ATP酶活性，揭示了MCM3在DNA复制起始中的调控机制。

4. **文献名称**：*"Expression of MCM3 in oral squamous cell carcinoma and its correlation with clinicopathological parameters"*

**作者**：Todorov et al.

**摘要**：该研究利用MCM3抗体检测口腔鳞癌组织，发现其表达水平与肿瘤侵袭性及淋巴结转移显著相关，支持MCM3作为口腔癌进展的预测因子。

这些文献涵盖了MCM3抗体在癌症诊断、增殖评估及分子机制研究中的应用。

       

背景信息

MCM3 (Minichromosome Maintenance Complex Component 3) is a critical protein involved in DNA replication and cell cycle regulation. As part of the hexameric MCM2-7 complex, it functions as a DNA helicase, unwinding double-stranded DNA during replication initiation. MCM3 plays a key role in ensuring precise genome duplication by restricting DNA replication to once per cell cycle. Dysregulation of MCM3 has been linked to uncontrolled cell proliferation, a hallmark of cancer, making it a potential biomarker for tumorigenesis.

Antibodies targeting MCM3 are widely used in research to study DNA replication mechanisms, cell cycle progression, and cancer biology. They enable detection of MCM3 expression and localization in cells or tissues via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Elevated MCM3 levels are often observed in malignancies, correlating with poor prognosis, which underscores its utility in cancer diagnostics and prognostic assessments.

Commercially available MCM3 antibodies are typically validated for specificity across human, mouse, and rat samples. Researchers also use them to explore interactions with other replication licensing factors (e.g., Cdc6. ORC) or to assess replication stress responses. As a conserved component of the replication machinery, MCM3 antibodies contribute to both basic science and translational studies aiming to target replication vulnerabilities in cancer therapy.

       
MCM3抗体;MCM3;MCM3 Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业，

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,蛋白组学,细胞生物学

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