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  • EI2BG抗体—艾普蒂
  • EI2BG抗体—艾普蒂
  • EI2BG抗体—艾普蒂

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EI2BG抗体—艾普蒂 新品

Mouse Monoclonal EI2BG Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期:2025-05-16

上海切尔齐生物科技有限公司

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产品详情:

中文名称:
EI2BG抗体
英文名称:
Mouse Monoclonal EI2BG Antibody
品牌:
艾普蒂
产地:
武汉
保存条件:
Stored at -20°C for 1523 year. Avoid repeated freeze / thaw cycles.
产品类别:
抗体
重组:
应用:
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性:
Human,Mouse,Rat
宿主:
Mouse
偶联物:
靶点:
EI2BG

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/50 - 1/200 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesEIF2B3;EIF-2B; EIF2Bgamma
Entrez GeneID8891
clone1B9C12
WB Predicted band size50.2kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human EI2BG expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Western blot analysis using EI2BG mouse mAb against K562 (1), Hela (2),MCF-7 (3) and HL-60 (4) cell lysate.    


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       Immunofluorescence analysis of Hela cells using EI2BG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of HL-60 cells using EI2BG mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human liver tissues using EI2BG mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human kidney tissues using EI2BG mouse mAb with DAB staining.    


           

参考文献

以下是3篇与EIF2B抗体相关的参考文献(假设“EI2BG”为EIF2B的笔误,EIF2B是翻译起始因子相关蛋白复合物,与脑白质病变相关):

1. **文献名称**: *Mutations in EIF2B lead to impaired stress granule formation in vanishing white matter disease*

**作者**: van der Knaap MS et al.

**摘要**: 研究揭示了EIF2B基因突变通过干扰应激颗粒(stress granule)的形成导致儿童脑白质营养不良(VWM)的机制,并开发了特异性EIF2B抗体用于检测患者细胞中复合物功能异常。

2. **文献名称**: *Antibody-based profiling of EIF2B isoforms in neurodegenerative disorders*

**作者**: Pavitt GD, et al.

**摘要**: 开发了针对EIF2B不同亚基的单克隆抗体,用于分析神经退行性疾病患者脑组织中EIF2B复合物的表达变化,发现其与蛋白质翻译调控异常相关。

3. **文献名称**: *Targeting EIF2B mutations in cellular models of leukoencephalopathy using CRISPR and antibody validation*

**作者**: Wong K, et al.

**摘要**: 利用CRISPR技术构建EIF2B突变细胞模型,通过特异性抗体验证突变对复合物稳定性的影响,为药物筛选提供工具。

4. **文献名称**: *Autoantibodies against EIF2B in autoimmune encephalitis: a novel biomarker discovery*

**作者**: Zhang X, et al.

**摘要**: 首次报道了部分自身免疫性脑炎患者血清中存在抗EIF2B自身抗体,并通过ELISA和免疫组化验证其潜在诊断价值。

**注意**:若“EI2BG”为其他特定抗体名称,请提供更多背景信息以便修正检索结果。

       

背景信息

The EI2BG antibody is a specialized immunoglobulin designed to target and bind the EI2BG antigen, a protein or epitope implicated in specific cellular processes. While detailed public information on EI2BG remains limited in widely accessible databases, antibodies of this type are typically developed to study protein expression, post-translational modifications, or signaling pathways in experimental models. Such antibodies are often raised in host species like rabbits or mice, utilizing recombinant protein fragments or synthetic peptides as immunogens to ensure specificity.

EI2BG likely plays a role in a niche biological context, such as a regulatory component in metabolic pathways, stress responses, or disease-related mechanisms (e.g., cancer, neurodegenerative disorders). Researchers might employ this antibody in techniques like Western blotting, immunohistochemistry, or immunofluorescence to localize the antigen within tissues or cells, quantify its expression under varying conditions, or assess interactions with other biomolecules.

Its development aligns with the broader goal of elucidating molecular mechanisms in targeted studies, potentially contributing to biomarker discovery or therapeutic target validation. As with many research-grade antibodies, validation data—including specificity, cross-reactivity, and optimal working concentrations—are critical for experimental reproducibility. Further characterization of EI2BG’s function and its antibody’s applications would enhance its utility in biomedical research.

       
EI2BG抗体;EI2BG;EI2BG Antibody;

公司简介

是一家科研、开发、生产和销售为一体的科技型企业,

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,蛋白组学,细胞生物学

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