H2AFX抗体—艾普蒂

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     Western blot analysis using H2AFX mouse mAb against HEK293 (1), HepG2 (2), Jurkat (3),Hela (4), Raji (5), K562 (6) and HCT116 (7) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using H2AFX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using H2AFX mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using H2AFX mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using H2AFX mouse mAb with DAB staining.    

  
  

The H2AFX antibody is a crucial tool in studying the DNA damage response and genomic stability. H2AFX, a variant of histone H2A, is rapidly phosphorylated at serine 139 (forming γH2AX) near DNA double-strand breaks (DSBs), a process mediated by kinases like ATM, ATR, or DNA-PK. This phosphorylation marks sites of DNA damage, recruiting repair proteins and facilitating chromatin remodeling. The γH2AX foci serve as biomarkers for DSBs, making H2AFX antibodies essential in detecting and quantifying DNA damage in cells.

Researchers use H2AFX antibodies in techniques such as immunofluorescence, Western blotting, and flow cytometry to assess genomic instability in cancer, aging, and radiation biology. These antibodies help evaluate the efficacy of DNA-damaging therapies (e.g., chemotherapy, radiotherapy) and study mechanisms of DNA repair pathways. Specificity for the phosphorylated form (γH2AX) is critical, as it distinguishes active damage signaling from baseline H2AFX. Commercial H2AFX antibodies are typically validated across human, mouse, and rat samples, though species reactivity should be confirmed. Proper controls, like irradiated cells (positive) or phosphatase-treated samples (negative), ensure accurate interpretation. Understanding H2AFX dynamics via these antibodies advances insights into diseases linked to genomic instability and therapeutic strategies targeting DNA repair.