SDHA抗体—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using SDHA mouse mAb against Jurkat (1), MCF-7 (2), Hela (3), HepG2 (4), PC-3 (5), .HL-60 (6),and NIH/3T3 (7) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using SDHA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Raji cells using SDHA mouse mAb (green) and negative control (red).    

  
  

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     Flow cytometric analysis of THP-1 cells using SDHA mouse mAb (green) and negative control (red).    

  
  

SDHA antibody targets the succinate dehydrogenase complex subunit A (SDHA), a key component of mitochondrial complex II involved in both the electron transport chain and the tricarboxylic acid (TCA) cycle. SDHA catalyzes the oxidation of succinate to fumarate, coupling this reaction with the reduction of ubiquinone. As a housekeeping protein, SDHA is widely expressed across tissues, making it a common loading control in Western blotting. However, its role extends beyond metabolism; SDHA mutations or dysfunction are linked to rare hereditary cancers (e.g., paragangliomas, gastrointestinal stromal tumors) and neurodegenerative disorders.

SDHA antibodies are essential tools for studying SDHA expression, localization, and interactions in disease models. They are used in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) to assess protein levels, mitochondrial integrity, or pathogenic variants. Validating these antibodies is critical due to potential cross-reactivity with other dehydrogenase subunits (e.g., SDHB) or isoforms. Researchers often confirm specificity using SDHA-knockout cell lines or siRNA knockdown. Commercial SDHA antibodies are typically raised in rabbits or mice against peptide sequences unique to the SDHA protein.

Recent studies highlight SDHA’s dual role as a metabolic enzyme and tumor suppressor, driving interest in its therapeutic targeting. Reliable SDHA antibodies are thus vital for advancing cancer research and mitochondrial disease diagnostics.