AKT1S1抗体—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using AKT1S1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using AKT1S1 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using AKT1S1 mouse mAb with DAB staining.    

  
  

The AKT1S1 antibody is a key tool for studying the AKT1 substrate 1 (AKT1S1), also known as PRAS40 (Proline-Rich AKT1 Substrate 1). AKT1S1 is a regulatory protein involved in the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway, which controls cell growth, proliferation, and metabolism. It acts as a scaffold linking mTORC1 to its downstream targets and is phosphorylated by AKT, enabling mTORC1 activation. Dysregulation of AKT1S1 is associated with cancers, metabolic disorders, and neurodegenerative diseases, making it a focus in biomedical research.

The AKT1S1 antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to detect AKT1S1 expression, post-translational modifications (e.g., phosphorylation at Thr246), and interactions with mTORC1 or other partners. Researchers rely on its specificity to distinguish AKT1S1 from homologous proteins and to study its role in cellular processes such as autophagy, apoptosis, and nutrient sensing. Commercial AKT1S1 antibodies are typically validated in multiple models, including human, mouse, and rat tissues. Understanding AKT1S1 dynamics through these antibodies provides insights into disease mechanisms and therapeutic targeting of the mTOR pathway.