WDFY3抗体—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Flow cytometric analysis of Hela cells using WDFY3 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using WDFY3 mouse mAb with DAB staining.    

  
  

The WDFY3 (WD repeat and FYVE domain-containing protein 3) antibody is a tool used to detect and study the WDFY3 protein, which plays a critical role in selective autophagy, particularly in autophagosome formation and cargo recognition. WDFY3 contains a FYVE domain that binds phosphatidylinositol 3-phosphate (PI3P) and WD40 repeats involved in protein interactions, enabling its function as a scaffold for recruiting autophagy-related proteins. Research has linked WDFY3 to neurodevelopmental disorders, including autism spectrum disorders (ASD), where mutations or dysregulation may impair synaptic pruning and neuronal connectivity. It is also implicated in cancer, with roles in tumor suppression by regulating apoptosis and cell proliferation in breast cancer, gliomas, and other malignancies.

The WDFY3 antibody is widely utilized in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and interactions in cellular and tissue samples. Antibodies targeting specific epitopes (e.g., rabbit or mouse monoclonal/polyclonal versions) are validated for specificity using knockout controls or siRNA knockdown. Studies employing these antibodies have advanced understanding of WDFY3’s involvement in autophagy-lysosomal pathways, neurodevelopment, and cancer biology. Its association with ASD and cancer highlights its potential as a biomarker or therapeutic target, driving interest in mechanistic and translational research.