RBBP7抗体—艾普蒂

+ +

•  

   

     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

•  

   

     Western blot analysis using RBBP7 mouse mAb against Jurkat (1), HepG2 (2), F9 (3), C6 (4), LNCAP (5), HL-60 (6), Hela (7), and SH-SY5Y (8) cell lysate.    

  
  

•  

   

     Immunofluorescence analysis of Hela cells using RBBP7 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.    

  
  

•  

   

     Immunofluorescence analysis of Hela cells using RBBP7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

•  

   

     Flow cytometric analysis of Hela cells using RBBP7 mouse mAb (green) and negative control (red).    

  
  

•  

   

     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using RBBP7 mouse mAb with DAB staining.    

  
  

•  

   

     Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using RBBP7 mouse mAb with DAB staining.    

  
  

**Background of RBBP7 Antibody**

RBBP7 (Retinoblastoma-Binding Protein 7), also known as RbAp46. is a member of the WD40 repeat protein family that plays a critical role in chromatin remodeling and epigenetic regulation. It interacts with histone deacetylases (HDACs) and polycomb group proteins, contributing to transcriptional repression and chromatin condensation. RBBP7 is a core component of several chromatin-modifying complexes, including the SIN3/HDAC, NuRD, and PRC2 complexes, which regulate gene expression during development, cell cycle progression, and differentiation.

Antibodies targeting RBBP7 are essential tools for studying its function in these processes. They are widely used in applications such as Western blotting, immunoprecipitation, and immunofluorescence to detect RBBP7 expression, localization, and protein-protein interactions. Research involving RBBP7 antibodies has linked the protein to cancer biology, as dysregulation of chromatin modifiers is common in malignancies. For example, RBBP7’s role in maintaining stem cell pluripotency and promoting tumorigenesis highlights its dual impact in normal physiology and disease.

These antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection. Both monoclonal and polyclonal RBBP7 antibodies are available, with selection depending on experimental needs. Understanding RBBP7’s regulatory mechanisms through such tools continues to advance insights into epigenetics, cancer therapy, and developmental biology.