TBP抗体—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using TBP mouse mAb against NIH/3T3 (1) and SK-N-SH (2) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using TBP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using TBP mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using TBP mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using TBP mouse mAb with DAB staining.    

  
  

**Background of TBP Antibodies**

TATA-box binding protein (TBP) is a critical transcription initiation factor that binds to the TATA-box DNA sequence in gene promoters, playing a central role in RNA polymerase II-mediated transcription. As a core component of the TFIID complex, TBP facilitates the assembly of the pre-initiation complex, enabling precise regulation of gene expression across eukaryotes. Its conserved structure, characterized by a saddle-shaped domain for DNA interaction, underscores its essential function in basal transcription.

TBP antibodies are immunological tools designed to detect, quantify, or isolate TBP in research and diagnostic applications. These antibodies, often monoclonal or polyclonal, are validated for techniques like Western blotting, chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and electrophoretic mobility shift assays (EMSAs). They help investigate TBP’s expression dynamics, interactions with transcriptional machinery, and alterations in diseases such as neurodegenerative disorders and cancers, where dysregulated transcription is implicated.

The development of TBP-specific antibodies has advanced studies on transcriptional regulation, cellular differentiation, and disease mechanisms. However, challenges remain in ensuring specificity, as TBP shares homology with related proteins (e.g., TBPL1/2). Rigorous validation and controls are thus essential for reliable data. Overall, TBP antibodies remain indispensable for dissecting the molecular underpinnings of gene expression and its pathologies.