CD127抗体—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using CD127 mouse mAb against Hela (1), HL-60 (2), and U937 (3) cell lysate.    

  
  

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     Flow cytometric analysis of HL-60 cells using CD127 mouse mAb (green) and negative control (red).    

  
  

CD127 antibody targets the interleukin-7 receptor alpha chain (IL-7Rα), a critical component of the IL-7 receptor complex (IL-7R), which pairs with the common gamma chain (γc) to mediate signaling. CD127 is expressed on immune cells, particularly naïve and memory T cells, regulatory T cells (Tregs), and innate lymphoid cells (ILCs). It plays a vital role in lymphocyte development, homeostasis, and immune responses by binding IL-7 and thymic stromal lymphopoietin (TSLP).

CD127 antibodies are widely used in research to identify and isolate T cell subsets. For example, CD127 is co-stained with markers like CD25 to distinguish activated effector T cells (CD25+CD127+) from immunosuppressive Tregs (CD25+CD127−/low). In flow cytometry, anti-CD127 helps characterize immune dysregulation in conditions like autoimmune diseases, infections, or cancers. Therapeutic applications are under exploration, as modulating IL-7/CD127 signaling may influence T cell survival or exhaustion. Some studies target this pathway to enhance antitumor immunity or mitigate autoimmunity.

Antibody clones vary in specificity (e.g., human: A019D5. mouse: SB/199). Validation ensures minimal cross-reactivity with related receptors. Challenges include distinguishing membrane-bound vs. soluble CD127 isoforms and addressing expression heterogeneity across cell types. Recent work also links CD127 polymorphisms to disease susceptibility, underscoring its clinical relevance.