APC2抗体—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using APC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Hela cells using APC2 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using APC2 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using APC2 mouse mAb with DAB staining.    

  
  

The APC2 (Adenomatous Polyposis Coli 2) antibody is a tool used to detect and study the APC2 protein, a member of the APC tumor suppressor family. APC2. also known as APCL, shares structural and functional similarities with the canonical APC protein (APC1), including β-catenin-binding domains critical for regulating the Wnt signaling pathway. While APC1 is prominently linked to colorectal cancer, APC2 plays a complementary role, particularly in tissues where APC1 is less expressed, such as the brain and developing embryos. Both proteins act as scaffolds to facilitate β-catenin degradation, thereby suppressing Wnt-driven transcriptional activity and maintaining cellular homeostasis.

APC2 antibodies are widely utilized in cancer research to investigate tumorigenesis, metastasis, and Wnt pathway dysregulation. They are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess APC2 expression levels, subcellular localization, and interactions with binding partners. Loss or mutation of APC2 has been implicated in various cancers, including medulloblastoma and hepatocellular carcinoma, often correlating with poor prognosis. Additionally, studies highlight its role in cytoskeletal organization, cell migration, and neuronal development.

These antibodies aid in elucidating APC2's tumor-suppressive mechanisms and its potential as a therapeutic target. Commercial APC2 antibodies are typically monoclonal or polyclonal, raised against specific epitopes, and validated for cross-reactivity and specificity to ensure reliable experimental outcomes.