WNK4抗体

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     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P12419(WNK4 Antibody) at dilution 1/45. (Original magnification: ×200)    

  
  

WNK4 (With No Lysine Kinase 4) is a serine/threonine kinase belonging to the WNK family, primarily involved in regulating ion transport pathways, particularly in the kidneys. It modulates the activity of ion cotransporters (e.g., NCC, NKCC2) and potassium channels via phosphorylation or protein-protein interactions, playing a critical role in maintaining electrolyte balance and blood pressure homeostasis. Dysregulation of WNK4 is linked to genetic hypertension disorders such as pseudohypoaldosteronism type II (PHAII), where mutations in the WNK4 gene cause abnormal renal sodium and potassium handling.

WNK4 antibodies are essential tools for studying its expression, localization, and function in physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect WNK4 protein levels in tissues, particularly in renal tubules. Research utilizing WNK4 antibodies has elucidated its role in cellular signaling networks, including interactions with SLC12A family transporters and the ubiquitin-proteasome system. Additionally, they aid in exploring WNK4's involvement in hypertension pathogenesis, drug responses (e.g., thiazide diuretics), and potential therapeutic targeting. Validation of antibody specificity remains crucial due to structural similarities among WNK kinases. Recent studies also employ WNK4 antibodies in transgenic animal models to dissect its tissue-specific effects on ion homeostasis and disease progression.