TRPV5抗体

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P12334(TRPV5 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

TRPV5 (Transient Receptor Potential Vanilloid 5) is a calcium-selective ion channel critical for maintaining systemic calcium homeostasis. Primarily expressed in the kidney, intestine, and placenta, it facilitates calcium reabsorption in the distal tubules of the nephron, playing a pivotal role in fine-tuning urinary calcium excretion. Dysregulation of TRPV5 has been implicated in hypercalciuria, kidney stone formation, and osteoporosis, making it a therapeutic target for calcium-related disorders.

TRPV5 antibodies are essential tools for studying the expression, localization, and function of this channel in physiological and pathological contexts. These antibodies are typically developed against specific epitopes of TRPV5. such as its intracellular N- or C-terminal regions, or extracellular loops. Validated through techniques like Western blotting, immunohistochemistry, and immunofluorescence, they help researchers detect TRPV5 protein levels in tissues or cultured cells.

In research, TRPV5 antibodies have been employed to investigate hormonal regulation (e.g., by vitamin D, parathyroid hormone) and interactions with calcium-binding proteins like calbindin-D28K. They also aid in exploring TRPV5's role in diseases, including renal calcium wasting syndromes and metabolic bone disorders. Specificity is confirmed using knockout models or siRNA-mediated knockdown. Both polyclonal and monoclonal TRPV5 antibodies are available, with selection depending on experimental needs. Their application continues to advance understanding of calcium transport mechanisms and potential therapeutic interventions.