RAB14抗体

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse brain tissue, Raji cells, Primary antibody: P12088(RAB14 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P12088(RAB14 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P12088(RAB14 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The RAB14 antibody is a crucial tool for studying the RAB14 protein, a member of the RAS superfamily of small GTPases. RAB14 regulates intracellular membrane trafficking, particularly in Golgi-to-plasma membrane transport, endocytic recycling, and maintaining organelle identity. It plays roles in diverse cellular processes, including cytokinesis, lipid droplet formation, and neuronal development. Dysregulation of RAB14 has been implicated in cancers, neurodegenerative diseases, and metabolic disorders.

RAB14 antibodies are typically generated using immunogenic peptides or recombinant proteins, available as monoclonal or polyclonal variants from hosts like rabbits or mice. These antibodies enable detection of RAB14 via techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Specific applications include mapping RAB14's subcellular localization, analyzing its expression levels in disease models, and studying interactions with effector proteins like RAB-interacting lysosomal protein (RILP).

Validation often involves knockdown/knockout controls or colocalization with organelle markers (e.g., Golgi or endosomal proteins). Commercial antibodies may vary in specificity, necessitating careful optimization for experimental conditions. Recent studies using RAB14 antibodies have uncovered its involvement in autophagy, pathogen invasion mechanisms, and drug resistance pathways, highlighting its therapeutic potential. Reliable RAB14 antibodies remain essential for elucidating its molecular functions and disease correlations.