RAB8A抗体

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human cerebella tissue, Human cerebrum tissue lysates, Primary antibody: P12086(RAB8A Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P12086(RAB8A Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

The RAB8A antibody is a crucial tool for studying the RAB8A protein, a member of the Ras-related small GTPase family involved in membrane trafficking and cellular dynamics. RAB8A regulates vesicular transport from the Golgi apparatus to the plasma membrane, playing a key role in exocytosis, ciliogenesis, and maintaining cell polarity. Dysregulation of RAB8A is linked to ciliopathies, neurodegenerative disorders (e.g., Parkinson’s disease), and cancer metastasis, making it a focus of pathophysiological research.

RAB8A antibodies, typically raised in rabbits or mice as monoclonal or polyclonal forms, enable the detection and localization of endogenous or exogenous RAB8A in techniques like Western blotting, immunofluorescence, and immunohistochemistry. These antibodies often target specific epitopes within conserved regions of RAB8A, such as its GTP-binding domains or C-terminal regions, allowing researchers to investigate its activation state (GTP- vs. GDP-bound) and interactions with effector proteins like optineurin or the exocyst complex.

Validated RAB8A antibodies are essential for studying its role in cellular processes, such as secretory vesicle trafficking, autophagy, and primary cilium formation. Commercial antibodies are rigorously tested for specificity (e.g., via knockout cell lines) and reproducibility, ensuring reliable data. Researchers also utilize these antibodies to explore disease mechanisms, such as impaired ciliary function in Joubert syndrome or RAB8A’s involvement in α-synuclein aggregation in Parkinson’s disease, underscoring their versatility in both basic and translational studies.