TTC12抗体

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Human bladder transitional cell carcinoma grade 2-3 tissue lysate, Primary antibody: P09945(TTC12 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P09945(TTC12 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The TTC12 antibody is a research tool designed to detect the TTC12 (tetratricopeptide repeat domain 12) protein, a component critical for cytoplasmic pre-assembly of dynein arms in motile cilia. TTC12 contains tetratricopeptide repeat (TPR) motifs, which mediate protein-protein interactions, and is part of a complex with DNAAF1 and DNAAF3. facilitating the cytoplasmic assembly of dynein motor proteins before their transport to cilia. Mutations in TTC12 are linked to primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, chronic respiratory infections, and infertility due to dysfunctional cilia and flagella.

TTC12 antibodies are typically produced in rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry to study TTC12 expression, localization, and interactions in cell lines or tissues. Specificity is often confirmed via knockout controls or siRNA knockdown. These antibodies have been instrumental in elucidating TTC12's role in dynein arm biogenesis and its association with ciliopathies. Recent studies also explore TTC12's potential involvement in sperm development and respiratory epithelial function, highlighting its broader relevance in cellular dynamics and disease mechanisms.