POLR1E抗体

The POLR1E antibody is designed to target the POLR1E protein, a key component of RNA polymerase I (Pol I). POLR1E, also known as AC19 or RPA49. is a conserved subunit of the Pol I complex, which is responsible for synthesizing ribosomal RNA (rRNA) in the nucleolus. This protein plays a critical role in transcription initiation and elongation, interacting with other Pol I subunits and regulatory factors to maintain ribosomal biogenesis—a process essential for cell growth and proliferation. Dysregulation of POLR1E has been implicated in diseases linked to disrupted ribosome production, such as certain cancers, ribosomopathies, and developmental disorders. Antibodies against POLR1E are widely used in research to investigate its expression, localization, and functional interactions. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation to study Pol I assembly, nucleolar organization, and stress-responsive transcriptional changes. Additionally, POLR1E antibodies aid in exploring its role in cellular responses to DNA damage or TP53-mediated pathways. These tools are often validated using knockout controls or recombinant protein assays to ensure specificity. Understanding POLR1E dynamics through such antibodies provides insights into ribosomal biology and potential therapeutic strategies targeting Pol I dysfunction in hyperproliferative diseases.