Recombinant Human IL-1a 重组人白细胞介素-1α

描述（Description）

IL-1 alpha is a ubiquitous and pivotal pro-inflammatory cytokine. IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha plays an important role in inflammation and bridges the innate and adaptive immune systems. IL-1 alpha mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways[1][4]. IL-1 alpha protein, Human is a recombinant protein consisting of 159 amino acids (S113-A271) and is produced by E. coli.

纯度（Purity）

≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining)

生物活性（Bioactivity）

Measured by its ability to induce NF-kB reporter activity by the reporter gene assay. TheED50  for this effect is ≤0.05ng/ml.

氨基酸序列Target Protein Sequence）

SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA

制剂（Trait）

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5.

复溶（Reconstitution）

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件&效期（Storage & Stability）

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输方式(Shipping)

Room temperature in continental US; may vary elsewhere.

生物活性（Bioactivity）

1.The ED50 is <1.0 pg/mL as measured by murine D10S cells, corresponding to a specific activity of >1.0 × 109 units/mg.
2.Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 10.95 pg/mL, corresponding to a specific activity is 91.32×106 units/mg.
3.Measured by its ability to down regulate the expression of CCN2 in NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20.03-24.05 pg/mL, corresponding to a specific activity is 4.158-4.992×107 U/mg.

Measured in a cell proliferation assay using CTLL-2 cells. The ED50 for this effect is 10.95 pg/mL，corresponding to a specific activity is 91.32×106 units/mg

电泳（SDS-PAGE）

Greater than 95% as determined by reducing SDS-PAGE.

特别提示：本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。