TLE1抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Jurkat, 231, A549 and A172 cell lysates, Primary antibody: P09798(TLE1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P09798(TLE1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P09798(TLE1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The TLE1 (Transducin-like enhancer of split 1) antibody is a tool used to detect the TLE1 protein, a member of the Groucho/Transducin-like enhancer of split (TLE) family. TLE1 functions as a transcriptional corepressor, regulating gene expression by interacting with transcription factors, such as those in the Wnt and Notch signaling pathways. Structurally, TLE1 contains an N-terminal Q domain for protein interactions and a C-terminal WD40 repeat domain critical for forming multiprotein complexes. It plays roles in embryonic development, cell differentiation, and tumorigenesis.

In diagnostic pathology, TLE1 antibodies are valuable for identifying synovial sarcoma, a soft-tissue malignancy. TLE1 is overexpressed in >95% of synovial sarcomas, making it a sensitive marker for distinguishing this tumor from histologically similar neoplasms. Immunohistochemistry (IHC) using TLE1 antibodies is routinely employed in clinical settings. However, specificity challenges exist, as TLE1 may also be expressed in other tumors, such as malignant peripheral nerve sheath tumors or solitary fibrous tumors, necessitating correlation with other markers (e.g., SS18-SSX fusion detection).

Research applications include studying TLE1's role in cancer progression, epigenetic regulation, and signaling pathways. Its involvement in repressing tumor suppressor genes or modulating oncogenic pathways like Hedgehog highlights its therapeutic relevance. Despite its utility, antibody performance can vary, emphasizing the need for validation in specific experimental or diagnostic contexts.