Recombinant Mouse LIF 重组小鼠白血病抑制因子

同用名/别名（Synonym）

Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA

描述（Description）

LIF Protein, Mouse is a lymphoid factor involved in various neural and inflammatory processes such as the acute-phase reaction, tissue damage, and infection.

纯度（Purity）

≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)

生物活性（Bioactivity）

1.The ED₅₀ is <0.01 ng/mL as measured by M1 cells, corresponding to a specific activity of >1.0 × 10⁸ units/mg.
2.Measured by its ability to induce IL-6 secretion by M-NFS-60 mouse cells. The ED₅₀ for this effect is 2.407 ng/mL, corresponding to a specific activity is 4.15×10⁵ U/mg.
3.Measured in a cell proliferation assay using C2C12 cell. The ED₅₀ for this effect is 0.3801-0.412 ng/mL.

氨基酸序列（Target Protein Sequence）

SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAFSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPF

制剂（Trait）

Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0 or 20mM PB, 150mM NaCl, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCL, pH 8.0 or PBS, pH 7.4, 8% trehalose.

复溶（Reconstitution）

It is recommended to redissolve in sterile deionized water.

保存条件&效期（Storage & Stability）

Stored at -20°C for 1 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.Please avoid repeated freeze-thaw cycles.

运输方式(Shipping)

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

生物活性（Bioactivity）

Measured in the M1 cell differentiation assay. The ED₅₀ is ≤0.2ng/ml.

电泳（SDS-PAGE）

Recombinant Mouse LIF  SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with  Fd004810  SDS-PAGE.

特别提示：本公司的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用。