EIF4A3抗体

The EIF4A3 antibody targets the eukaryotic initiation factor 4A-III (EIF4A3), a member of the DEAD-box RNA helicase family involved in RNA metabolism. EIF4A3 is a core component of the exon junction complex (EJC), which forms during pre-mRNA splicing and plays critical roles in mRNA surveillance, localization, and translation. Unlike its paralogs EIF4A1 and EIF4A2. EIF4A3 lacks direct involvement in canonical translation initiation but is essential for nonsense-mediated mRNA decay (NMD), a quality-control mechanism that degrades aberrant transcripts. Structurally, EIF4A3 binds ATP and RNA, utilizing helicase activity to remodel RNA-protein interactions within the EJC. Antibodies against EIF4A3 are widely used to study its function in RNA processing, EJC assembly, and NMD pathways. They are employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect protein expression, localization, and interactions. Dysregulation of EIF4A3 is linked to cancers and neurological disorders, making its antibody a valuable tool for investigating disease mechanisms. Research also explores EIF4A3's role in cellular stress responses and viral infections, highlighting its broader significance in post-transcriptional gene regulation.