NOSIP抗体

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09647(NOSIP Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P09647(NOSIP Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

NOSIP (Nitric Oxide Synthase Interacting Protein) is a cellular protein first identified in the early 2000s as a binding partner of neuronal nitric oxide synthase (nNOS). It belongs to a family of NOS-interacting proteins that regulate the activity, localization, and stability of nitric oxide synthase (NOS) isoforms, which produce nitric oxide (NO)—a key signaling molecule involved in vascular tone, neurotransmission, and immune responses. NOSIP is ubiquitously expressed and interacts with both nNOS and endothelial NOS (eNOS), though its functional roles extend beyond NO regulation.

Studies suggest NOSIP modulates NOS trafficking by promoting the translocation of eNOS from the plasma membrane to intracellular compartments, thereby reducing NO production. It also participates in diverse cellular processes, including protein ubiquitination, cell cycle regulation, and apoptosis, through interactions with proteins like cullin-3 (a component of E3 ubiquitin ligase complexes). Dysregulation of NOSIP has been linked to pathological conditions such as cardiovascular diseases, cancer, and neurological disorders. For instance, altered NOSIP expression has been observed in tumor tissues, potentially influencing cancer progression via NO-dependent pathways.

NOSIP-specific antibodies are critical tools for investigating these mechanisms. They enable detection of NOSIP expression levels, subcellular localization, and protein-protein interactions in experimental models, aiding research into its physiological and pathological roles. Commercial antibodies are typically validated for applications like Western blotting, immunohistochemistry, and co-immunoprecipitation.