CYP2E1抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A375 and Jurkat cell lysates, Primary antibody: P02481(SLC3A2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P02481(SLC3A2 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P02481(SLC3A2 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

CYP2E1 (Cytochrome P450 2E1) is a member of the cytochrome P450 enzyme superfamily, primarily expressed in the liver and involved in the metabolism of endogenous compounds and xenobiotics. It plays a critical role in oxidizing small hydrophobic molecules, including ethanol, acetaminophen, and industrial solvents, often generating reactive oxygen species (ROS) that contribute to cellular oxidative stress. CYP2E1 is highly inducible by alcohol, obesity, and diabetes, making it a key focus in studying alcohol-induced liver injury, drug toxicity, and metabolic disorders.

Antibodies targeting CYP2E1 are essential tools for detecting its expression and activity in tissues or cell cultures. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to investigate CYP2E1's role in disease mechanisms. For instance, elevated CYP2E1 levels are linked to alcoholic liver disease, non-alcoholic fatty liver disease (NAFLD), and certain cancers. Researchers also employ these antibodies to study interactions between CYP2E1 and other proteins or signaling pathways, as well as its regulation under varying physiological or pathological conditions.

Commercial CYP2E1 antibodies are typically raised against specific epitopes of human or rodent CYP2E1 proteins. However, variability in antibody specificity and cross-reactivity across species requires careful validation. Studies using these antibodies have revealed tissue-specific expression patterns and underscored CYP2E1's dual role as both a detoxifying enzyme and a contributor to oxidative damage, highlighting its therapeutic and diagnostic relevance in metabolic and toxicological research.