CANX抗体 新品

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: Hela cells, 231 cells, human placenta tissue, Human carcinoma of sigmoid tissue, Primary antibody: P08098(CANX Antibody) at dilution 1/615, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P08098(CANX Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain  tissue using P08098(CANX Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

**Background of CANX Antibodies**

CANX (Calnexin) is a 90 kDa chaperone protein primarily located in the endoplasmic reticulum (ER) membrane. It plays a critical role in the quality control of glycoprotein folding by binding to monoglucosylated N-linked glycans, ensuring proper folding and assembly of nascent proteins. Calnexin also interacts with ERp57. a thiol-oxidoreductase, to facilitate disulfide bond formation. Additionally, it participates in the ER-associated degradation (ERAD) pathway, targeting misfolded proteins for proteasomal degradation.

CANX antibodies are widely used in research to study ER stress, protein folding dynamics, and diseases linked to proteostasis dysfunction, such as neurodegenerative disorders, diabetes, and cancer. These antibodies are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect Calnexin expression, localization, and interactions.

Most CANX antibodies are raised against conserved epitopes, enabling cross-reactivity across species (human, mouse, rat). Validation includes verifying specificity via knockout cell lines or siRNA knockdown. Due to its ER retention signal (KDEL-like motif), Calnexin is also used as an ER marker in subcellular localization studies. Researchers must consider potential cross-reactivity with calreticulin, a soluble homolog, when interpreting results.